Molecular cloning and expression analysis of a heat shock protein (Hsp90) gene from black tiger shrimp (Penaeus monodon)

The techniques of homology cloning and anchored PCR were used to clone the Hsp90 gene from black tiger shrimp. The full length cDNA of black tiger shrimp Hsp90 (btsHsp90) contained a 5' untranslated region (UTR) of 72 bp, an ORF (open reading frame) of 2160 bp encoding a polypeptide of 720 amin...

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Bibliographic Details
Published in:Molecular biology reports 2009-01, Vol.36 (1), p.127-134
Main Authors: Jiang, Shigui, Qiu, Lihua, Zhou, Falin, Huang, Jianhua, Guo, Yihui, Yang, Keng
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animal Anatomy
Animal Biochemistry
Animals
Base Sequence
Biomedical and Life Sciences
Chiromantes haematocheir
Cloning
Cloning, Molecular
Gene expression
Gene Expression Profiling
Heat shock proteins
Histology
HSP90 Heat-Shock Proteins - chemistry
HSP90 Heat-Shock Proteins - genetics
HSP90 Heat-Shock Proteins - metabolism
Life Sciences
Marine
Molecular biology
Molecular Sequence Data
Morphology
Penaeidae - genetics
Penaeus monodon
Phylogeny
Polymerase chain reaction
Reverse Transcriptase Polymerase Chain Reaction
Sequence Alignment
Sequence Homology, Nucleic Acid
Shellfish
Tissue Array Analysis
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Summary:The techniques of homology cloning and anchored PCR were used to clone the Hsp90 gene from black tiger shrimp. The full length cDNA of black tiger shrimp Hsp90 (btsHsp90) contained a 5' untranslated region (UTR) of 72 bp, an ORF (open reading frame) of 2160 bp encoding a polypeptide of 720 amino acids with an estimated molecular mass of 83-kDa and a 3' UTR of 288 bp. The sequence of the coding region showed 90 and 84% homology with that of the Chiromantes haematocheir and Homo sapiens, respectively. Conserved signature sequences of Hsp90 gene family were found in the btsHsp90 deduced amino acid sequence. The temporal expressions of Hsp90 gene were constitutively in the black tiger shrimp tissues including liver, ovary, muscle, brain stomach, and heart, and their levels were markedly enhanced after 30-min heat treatment at 37°C. In ovarian maturation stages, the expression of btsHsp90 was strongest in the second stage, weaker in the fourth and first stage.
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-007-9160-9