Human cytomegalovirus persists in myeloid progenitors and is passed to the myeloid progeny in a latent form

Summary CD34+ progenitor cells can harbour latent human cytomegalovirus (HCMV); however, the mechanisms of HCMV latency remain unclear. We have investigated the effects of the haematopoietic lineage restriction on the establishment and spread of the latent HCMV to progeny cells. In vitro‐infected an...

Full description

Saved in:
Bibliographic Details
Published in:British journal of haematology 2004-08, Vol.126 (3), p.410-417
Main Authors: Khaiboullina, Svetlana F., Maciejewski, Jaroslaw P., Crapnell, Kirsten, Spallone, Patricia A., Dean Stock, A., Pari, Gregory S., Zanjani, Esmail D., Jeor, Stephen St
Format: Article
Language:English
Subjects:
Antibodies, Viral - analysis
Antigens, CD34
Biological and medical sciences
Bone Marrow Cells - virology
bone marrow progenitor cells
Cell Division
Cells, Cultured
cytomegalovirus
Cytomegalovirus - genetics
Cytomegalovirus - immunology
Cytomegalovirus - physiology
Cytomegalovirus Infections - transmission
DNA, Viral - analysis
Fibroblasts - virology
Hematologic and hematopoietic diseases
Hematology
Humans
Image Processing, Computer-Assisted
In Situ Hybridization - methods
latency
Male
Medical sciences
Microscopy, Immunoelectron
Stem Cells - virology
Virus Latency
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Summary CD34+ progenitor cells can harbour latent human cytomegalovirus (HCMV); however, the mechanisms of HCMV latency remain unclear. We have investigated the effects of the haematopoietic lineage restriction on the establishment and spread of the latent HCMV to progeny cells. In vitro‐infected and latently‐infected haematopoietic progenitor cells derived from HCMV seropositive donors were studied. The presence of HCMV DNA in bone marrow progenitor (BMP) cells was determined by single colony polymerase chain reaction and fluorescent in situ hybridization (FISH). The presence of CMV DNA was found to be restricted to myeloid progenitors and the percentage of HCMV‐infected cells was lower in naturally‐infected cells than in in vitro‐infected cells. Erythroid differentiation resulted in an abortive infection with persistence of the viral nucleic acids in red cell precursors. In BMP cells from HCMV seronegative donors, HCMV DNA was localized in the nucleus. Bone marrow progenitors in the presence of granulocyte‐macrophage colony stimulating factor (GMCSF) maintained HCMV DNA for extended periods of time. No viral production could be detected throughout the culture but the comparison of the numbers of latently‐infected cells prior to and after the culture suggests that proliferation of haematopoietic progenitor cells may lead to the expansion of latently‐infected cells.
ISSN:0007-1048
1365-2141
DOI:10.1111/j.1365-2141.2004.05056.x