The PI(3,5)P2 and PI(4,5)P2 Interactomes

A comprehensive analysis of the phosphoinositide interactome has been performed using analogues of PI(3,5)P2 and PI(4,5)P2 phosphatidyl phospholipids which were immobilized onto Affi-10 beads or incorporated into liposomes for use as affinity absorbents with cytosolic extracts from colonic carcinoma...

Full description

Saved in:
Bibliographic Details
Published in:Journal of proteome research 2008-12, Vol.7 (12), p.5295-5313
Main Authors: Catimel, Bruno, Schieber, Christine, Condron, Melanie, Patsiouras, Heather, Connolly, Lisa, Catimel, Jenny, Nice, Edouard C, Burgess, Antony W, Holmes, Andrew B
Format: Article
Language:English
Subjects:
Cell Line, Tumor
Chromatography, Ion Exchange - methods
Cytosol - metabolism
Electrophoresis, Polyacrylamide Gel
Gene Expression Regulation, Enzymologic
Humans
Liposomes - chemistry
Magnetic Resonance Spectroscopy
Peptides - chemistry
Phosphatidylinositol 4,5-Diphosphate - chemistry
Phosphatidylinositol 4,5-Diphosphate - metabolism
Phosphatidylinositol Phosphates - chemistry
Phosphatidylinositol Phosphates - metabolism
Phospholipids - chemistry
Phosphorylation
Proteome
Proteomics - methods
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A comprehensive analysis of the phosphoinositide interactome has been performed using analogues of PI(3,5)P2 and PI(4,5)P2 phosphatidyl phospholipids which were immobilized onto Affi-10 beads or incorporated into liposomes for use as affinity absorbents with cytosolic extracts from colonic carcinoma cell lines. Affinity/LC/MS/MS experiments allowed identification of 388 proteins/protein complexes that appeared to interact specifically with the phosphoinositide targets: a number of novel potential phosphoinositide interacting proteins have been identified.
ISSN:1535-3893
1535-3907
DOI:10.1021/pr800540h