Serological and molecular detection of Borrelia burgdorferi sensu lato and Anaplasmataceae in rodents

The presence of Anaplasma spp. and Borrelia burgdorferi sensu lato in rodents from Eastern Slovakia were followed by serological and molecular methods. The seroprevalence for Borrelia was detected in 16.6%, for Anaplasmataceae (APT) in 13.2% and co-occurrence of Borrelia and APT in 7.5%. Out of 110...

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Published in:Folia microbiologica 2008-01, Vol.53 (6), p.493-499
Main Authors: Stefancikova, A.,Slovenska Akademia Vied, Kosice (Slovak Republic). Parasitologicky Ustav, Derdakova, M.,Slovenska Akademia Vied, Kosice (Slovak Republic). Parasitologicky Ustav, Lencakova, D.,Slovenska Akademia Vied, Kosice (Slovak Republic). Parasitologicky Ustav, Ivanova, R.,Slovenska Akademia Vied, Kosice (Slovak Republic). Parasitologicky Ustav, Stanko, M.,Slovenska Akademia Vied, Kosice (Slovak Republic). Ustav Zoologie, Cislakova, L.,Univerzita Pavla Josefa Safarika, Kosice (Slovak Republic). Lekarska Fakulta, Petko, B.,Slovenska Akademia Vied, Kosice (Slovak Republic). Parasitologicky Ustav
Format: Article
Language:English
Subjects:
AGENT PATHOGENE
ANALISIS MICROBIOLOGICO
ANALYSE MICROBIOLOGIQUE
ANAPLASMA
Anaplasmataceae - genetics
Anaplasmataceae - immunology
Anaplasmataceae - isolation & purification
Anaplasmataceae Infections - diagnosis
Anaplasmataceae Infections - epidemiology
Anaplasmataceae Infections - microbiology
Anaplasmataceae Infections - veterinary
Animals
ANTIBODIES
Antibodies, Bacterial - blood
ANTICORPS
ANTICUERPOS
Applied Microbiology
Arvicolinae - microbiology
Bacteria
Biomedical and Life Sciences
BIOPSIA
BIOPSIE
BIOPSY
BORRELIA BURGDORFERI
Borrelia burgdorferi Group - isolation & purification
Disease Reservoirs
DNA, Bacterial - genetics
DNA, Bacterial - isolation & purification
Ear, External - microbiology
ELISA
Environmental Engineering/Biotechnology
Enzyme-Linked Immunosorbent Assay
Epidemiology
http://www.fao.org/aos/agrovoc#c_13948
http://www.fao.org/aos/agrovoc#c_15968
http://www.fao.org/aos/agrovoc#c_16102
http://www.fao.org/aos/agrovoc#c_25302
http://www.fao.org/aos/agrovoc#c_27555
http://www.fao.org/aos/agrovoc#c_33096
http://www.fao.org/aos/agrovoc#c_33630
http://www.fao.org/aos/agrovoc#c_388
http://www.fao.org/aos/agrovoc#c_493
http://www.fao.org/aos/agrovoc#c_5630
http://www.fao.org/aos/agrovoc#c_6602
IMMUNODIAGNOSIS
IMMUNODIAGNOSTIC
Immunology
INMUNODIAGNOSTICO
Life Sciences
Lyme Disease - diagnosis
Lyme Disease - epidemiology
Lyme Disease - microbiology
Lyme Disease - veterinary
Mice
MICROBIOLOGICAL ANALYSIS
Microbiology
Molecular biology
Molecular Sequence Data
Murinae - microbiology
ORGANISMOS PATOGENOS
Parasitic diseases
PATHOGENS
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Reproducibility of Results
REPUBLICA ESLOVACA
REPUBLIQUE SLOVAQUE
RICKETTSIALES
Rodent Diseases - diagnosis
Rodent Diseases - epidemiology
Rodent Diseases - microbiology
RODENTS
ROEDORES
RONGEUR
Sequence Alignment
Seroepidemiologic Studies
SLOVAK REPUBLIC
Slovakia - epidemiology
TEST ELISA
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Description
Summary:The presence of Anaplasma spp. and Borrelia burgdorferi sensu lato in rodents from Eastern Slovakia were followed by serological and molecular methods. The seroprevalence for Borrelia was detected in 16.6%, for Anaplasmataceae (APT) in 13.2% and co-occurrence of Borrelia and APT in 7.5%. Out of 110 ear biopsies of rodents, 5 were B. afzelii-positive. Five biopsies tested positive with the Ehr521-Ehr747 primers amplifying all the members of the family APT. A. phagocytophilum was detected in 1.8% of samples, 2.7% were infected with Anaplasma-like organisms. Co-occurrence of Borrelia and Anaplasma in ear biopsies was found in 1.8%. The circulation of both Borrelia and Anaplasma in the region of Eastern Slovakia was thus confirmed.
ISSN:0015-5632
1874-9356
DOI:10.1007/s12223-008-0077-z