The effects of lycopene on the proliferation of human breast cells and BRCA1 and BRCA2 gene expression

The purpose of this study was to demonstrate the effects of lycopene, the major tomato carotenoid, on the expression of the BRCA1 and BRCA2 genes in three breast tumour cell lines, MCF-7, HBL-100, MDA-MB-231 and the fibrocystic breast cell line MCF-10a. Flow cytometry analysis showed a G 1/S phase c...

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Bibliographic Details
Published in:European journal of cancer (1990) 2004-07, Vol.40 (11), p.1768-1775
Main Authors: Chalabi, N, Le Corre, L, Maurizis, J-C, Bignon, Y-J, Bernard-Gallon, D.J
Format: Article
Language:English
Subjects:
Affinity chromatography
Base Sequence
Biological and medical sciences
BRCA1
BRCA2
Breast cancer
Breast Neoplasms - genetics
Breast Neoplasms - pathology
Carotenoids - pharmacology
Cell Division - drug effects
DNA, Complementary - metabolism
Female
Genes, BRCA1
Genes, BRCA2
Gynecology. Andrology. Obstetrics
Humans
Lycopene
Mammary gland diseases
Medical sciences
Pharmacology. Drug treatments
Protein quantification
Quantitative RT-PCR
Reverse Transcriptase Polymerase Chain Reaction - methods
RNA, Neoplasm - metabolism
Tumors
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Summary:The purpose of this study was to demonstrate the effects of lycopene, the major tomato carotenoid, on the expression of the BRCA1 and BRCA2 genes in three breast tumour cell lines, MCF-7, HBL-100, MDA-MB-231 and the fibrocystic breast cell line MCF-10a. Flow cytometry analysis showed a G 1/S phase cell cycle-arrest after treatment of the cells with 10 μM lycopene for 48 h. mRNA expression was studied by quantitative reverse transcription-polymerase chain reaction using the Taqman ® method. We observed an increase of BRCA1 and BRCA2 mRNA in the oestrogen receptor (ER)-positive cell lines (MCF-7 and HBL-100), and a decrease (MDA-MB-231) or no change (MCF-10a) in the ER-negative cell lines. BRCA1 and BRCA2 proteins were quantified by perfusion affinity chromatography. No variation in their expression was observed. These preliminary results on the effects of lycopene on the expression of BRCA1 and BRCA2 oncosuppressor genes in breast cancer may reflect cross-talk between the oestrogen and retinoic acid receptor (RAR) pathways.
ISSN:0959-8049
1879-0852
DOI:10.1016/j.ejca.2004.03.028