tomato homolog of the gene encoding UV-damaged DNA binding protein 1 (DDB1) underlined as the gene that causes the high pigment-1 mutant phenotype

A tomato EST sequence, highly homologous to the human and Arabidopsis thaliana UV-damaged DNA binding protein 1 (DDB1), was mapped to the centromeric region of the tomato chromosome 2. This region was previously shown to harbor the HP-1 gene, encoding the high pigment-1 (hp-1) and the high pigment-1...

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Bibliographic Details
Published in:Theoretical and applied genetics 2004-05, Vol.108 (8), p.1574-1581
Main Authors: Lieberman, M, Segev, O, Gilboa, N, Lalazar, A, Levi, I
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acid sequences
Arabidopsis thaliana
Base Sequence
Biological and medical sciences
chromosome mapping
Chromosomes, Plant - genetics
Classical genetics, quantitative genetics, hybrids
Cloning, Molecular
complementary DNA
cultivars
DDB1 gene
DNA-binding proteins
DNA-Binding Proteins - chemistry
DNA-Binding Proteins - genetics
Fundamental and applied biological sciences. Psychology
Genes, Plant - genetics
Genetic Linkage - genetics
genetic markers
Genetics
Genetics of eukaryotes. Biological and molecular evolution
Genotype
Hypocotyl - genetics
Hypocotyl - physiology
hypocotyl length
hypocotyls
lines
linkage (genetics)
Lycopersicon esculentum
Lycopersicon esculentum - classification
Lycopersicon esculentum - genetics
Lycopersicon esculentum - physiology
Molecular Sequence Data
morphogenesis
mutants
Mutation
Mutation - genetics
nucleotide sequences
Phenotype
photomorphogenesis
Polymorphism, Single Nucleotide - genetics
Pteridophyta, spermatophyta
seedling growth
seedlings
Sequence Analysis, DNA
Sequence Homology
single nucleotide polymorphism
Solanum lycopersicum var. lycopersicum
structural genes
tomatoes
Vegetals
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Summary:A tomato EST sequence, highly homologous to the human and Arabidopsis thaliana UV-damaged DNA binding protein 1 (DDB1), was mapped to the centromeric region of the tomato chromosome 2. This region was previously shown to harbor the HP-1 gene, encoding the high pigment-1 (hp-1) and the high pigment-1w (hp-1w) mutant phenotypes. Recent results also show that the A. thaliana DDB1 protein interacts both genetically and biochemically with the protein encoded by DEETIOLATED1, a gene carrying three tomato mutations that are in many respects isophenotypic to hp-1: high pigment-2 (hp-2), high pigment-2j (hp-2j) and dark green (dg). The entire coding region of the DDB1 gene was sequenced in an hp-1 mutant and its near-isogenic normal plant in the cv. Ailsa Craig background, and also in an hp-1w mutant and its isogenic normal plant in the GT breeding line background. Sequence analysis revealed a single A931-to-T931 base transversion in the coding sequence of the DDB1 gene in the hp-1 mutant plants. This transversion results in the substitution of the conserved asparagine at position 311 to a tyrosine residue. In the hp-1w mutant, on the other hand, a single G2392-to-A2392 transition was observed, resulting in the substitution of the conserved glutamic acid at position 798 to a lysine residue. The single nucleotide polymorphism that differentiates hp-1 mutant and normal plants in the cv. Ailsa Craig background was used to design a pyrosequencing genotyping system. Analysis of a resource F2 population segregating for the hp-1 mutation revealed a very strong linkage association between the DDB1 locus and the photomorphogenic response of the seedlings, measured as hypocotyl length (25
ISSN:0040-5752
1432-2242
DOI:10.1007/s00122-004-1584-1