Identification of survivin, an inhibitor of apoptosis, in canine urinary bladder transitional cell carcinoma

Survivin, an inhibitor of apoptosis, is overexpressed in human invasive transitional cell carcinoma (TCC) of the urinary bladder. Survivin expression in canine TCC has not been defined. This study was designed to compare survivin expression between canine TCC and normal urinary bladder tissue. Rever...

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Bibliographic Details
Published in:Veterinary & comparative oncology 2008-09, Vol.6 (3), p.141-150
Main Authors: Rankin, W. Velando, Henry, C.J, Turnquist, S.E, Turk, J.R, Beissenherz, M.E, Tyler, J.W, Rucker, E.B, Knapp, D.W, Rodriguez, C.O, Green, J.A
Format: Article
Language:English
Subjects:
Animals
apoptosis
Apoptosis - physiology
bladder
bladder diseases
carcinoma
Carcinoma, Transitional Cell - metabolism
Carcinoma, Transitional Cell - veterinary
cell nucleus
cytoplasm
dog
dog diseases
Dog Diseases - metabolism
Dogs
Female
Gene Expression Regulation, Neoplastic
immunohistochemistry
Male
messenger RNA
metabolic inhibitors
Microtubule-Associated Proteins - metabolism
Reverse Transcriptase Polymerase Chain Reaction - veterinary
survivin
transitional cell carcinoma
Urinary Bladder Neoplasms - metabolism
Urinary Bladder Neoplasms - veterinary
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Summary:Survivin, an inhibitor of apoptosis, is overexpressed in human invasive transitional cell carcinoma (TCC) of the urinary bladder. Survivin expression in canine TCC has not been defined. This study was designed to compare survivin expression between canine TCC and normal urinary bladder tissue. Reverse-transcriptase polymerase chain reaction (PCR) and immunohistochemistry (IHC) were performed on fresh-frozen and formalin-fixed tissues, respectively. All TCC tissues (n = 6) and 11/22 normal tissues assessed by PCR were positive for survivin. This difference was not significant (P = 0.06). With regard to IHC, 28/41 TCC samples were positive for nuclear survivin, whereas 0/46 normal tissues had nuclear immunoreactivity (P < 0.001). Cytoplasmic immunoreactivity did not significantly differ between TCC (7/41) and normal tissues (17/46) (P = 0.07). We conclude that nuclear survivin is present in canine TCC, but not in normal bladder urothelium. Future studies will evaluate the role of nuclear survivin in TCC development and as a potential therapeutic target.
ISSN:1476-5810
1476-5829
DOI:10.1111/j.1476-5829.2007.00150.x