Enzymatic production of [alpha]-d-galactose 1-phosphate by lactose phosphorolysis

Enzymatic production of [alpha]-d-galactose 1-phosphate by lactose phosphorolysis

a-d-Galactose 1-phosphate (aGal1P) is an important building block for the synthesis of nucleotide sugars that are substrates for glycosyltransferases. We have previously reported the creation of novel lactose phosphorylase enzymes that are useful for the synthesis of aGal1P from the cheap and abunda...

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Published in:Biotechnology letters 2009-12, Vol.31 (12), p.1873-1877
Main Authors: Groeve, Manu RM, Depreitere, Veerle, Desmet, Tom, Soetaert, Wim
Format: Article
Language:eng
Subjects:
Biotechnology
Chemical synthesis
Chromatography
Enzymes
Escherichia coli
Ethyl alcohol
Lactose
Nucleotides
Phosphorylases
Precipitation
Synthesis
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recordid cdi_proquest_miscellaneous_36344635
title Enzymatic production of [alpha]-d-galactose 1-phosphate by lactose phosphorolysis
format Article
creator Groeve, Manu RM
Depreitere, Veerle
Desmet, Tom
Soetaert, Wim
subjects Biotechnology
Chemical synthesis
Chromatography
Enzymes
Escherichia coli
Ethyl alcohol
Lactose
Nucleotides
Phosphorylases
Precipitation
Synthesis
ispartof Biotechnology letters, 2009-12, Vol.31 (12), p.1873-1877
description a-d-Galactose 1-phosphate (aGal1P) is an important building block for the synthesis of nucleotide sugars that are substrates for glycosyltransferases. We have previously reported the creation of novel lactose phosphorylase enzymes that are useful for the synthesis of aGal1P from the cheap and abundant lactose. Here we describe the application of such a lactose phosphorylase in a production system using permeabilized Escherichia coli cells. After purification of the product from the reaction mixture by anion-exchange chromatography and ethanol precipitation, 9.5 grams of highly pure aGal1P were obtained from a 1l reaction volume.
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1573-6776
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