Enzymatic production of [alpha]-d-galactose 1-phosphate by lactose phosphorolysis

a-d-Galactose 1-phosphate (aGal1P) is an important building block for the synthesis of nucleotide sugars that are substrates for glycosyltransferases. We have previously reported the creation of novel lactose phosphorylase enzymes that are useful for the synthesis of aGal1P from the cheap and abunda...

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Bibliographic Details
Published in:	Biotechnology letters 2009-12, Vol.31 (12), p.1873-1877
Main Authors:	Groeve, Manu RM, Depreitere, Veerle, Desmet, Tom, Soetaert, Wim
Format:	Article
Language:	eng
Subjects:	Biotechnology Chemical synthesis Chromatography Enzymes Escherichia coli Ethyl alcohol Lactose Nucleotides Phosphorylases Precipitation Synthesis
Online Access:	Get full text
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Summary:	a-d-Galactose 1-phosphate (aGal1P) is an important building block for the synthesis of nucleotide sugars that are substrates for glycosyltransferases. We have previously reported the creation of novel lactose phosphorylase enzymes that are useful for the synthesis of aGal1P from the cheap and abundant lactose. Here we describe the application of such a lactose phosphorylase in a production system using permeabilized Escherichia coli cells. After purification of the product from the reaction mixture by anion-exchange chromatography and ethanol precipitation, 9.5 grams of highly pure aGal1P were obtained from a 1l reaction volume.
ISSN:	0141-5492 1573-6776