A Mediator-Free Bienzyme Amperometric Biosensor Based on Horseradish Peroxidase and Glucose Oxidase Immobilized on Carbon Nanotube Modified Electrode

Multiwalled carbon nanotube (CNT) modified glassy carbon electrode immobilized with horseradish peroxidase (HRP) in Nafion coating showed direct electron transfer between HRP enzyme and the CNT‐modified electrode. A mediator‐free bienzyme glucose biosensor based on horseradish peroxidase and glucose...

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Bibliographic Details
Published in:Electroanalysis (New York, N.Y.) N.Y.), 2008-10, Vol.20 (19), p.2090-2095
Main Authors: Yao, Yan-Li, Shiu, Kwok-Keung
Format: Article
Language:English
Subjects:
Bienzyme glucose biosensor
Carbon nanotubes
Direct electrochemistry
Glucose oxidase
Horseradish peroxidase
Mediator-free
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Summary:Multiwalled carbon nanotube (CNT) modified glassy carbon electrode immobilized with horseradish peroxidase (HRP) in Nafion coating showed direct electron transfer between HRP enzyme and the CNT‐modified electrode. A mediator‐free bienzyme glucose biosensor based on horseradish peroxidase and glucose oxidase was constructed. The bienzyme biosensor exhibited a high sensitivity for glucose detection at zero applied potential.
ISSN:1040-0397
1521-4109
DOI:10.1002/elan.200804297