Lipase-catalyzed ethanolysis of soybean oil in a solvent-free system using central composite design and response surface methodology

BACKGROUND: In this work we describe the synthesis of ethyl esters, commonly known as biodiesel, using refined soybean oil and ethanol in a solvent-free system catalyzed by lipase from Thermomyces lanuginosus. Central composite design and response surface methodology (RSM) were employed to optimize...

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Bibliographic Details
Published in:Journal of chemical technology and biotechnology (1986) 2008-06, Vol.83 (6), p.849-854
Main Authors: Rodrigues, Rafael Costa, Volpato, Giandra, Ayub, Marco Antônio Záchia, Wada, Keiko
Format: Article
Language:English
Subjects:
Applied sciences
biodiesel
Biological and medical sciences
Biotechnology
Catalysis
Catalytic reactions
Chemical engineering
Chemistry
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
General and physical chemistry
lipase
Methods. Procedures. Technologies
optimization
Others
Reactors
response surface methodology
solvent-free system
Theory of reactions, general kinetics. Catalysis. Nomenclature, chemical documentation, computer chemistry
Thermomyces lanuginosus
Various methods and equipments
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Summary:BACKGROUND: In this work we describe the synthesis of ethyl esters, commonly known as biodiesel, using refined soybean oil and ethanol in a solvent-free system catalyzed by lipase from Thermomyces lanuginosus. Central composite design and response surface methodology (RSM) were employed to optimize the biodiesel synthesis parameters, which were: reaction time, temperature, substrate molar ratio, enzyme content, and added water, measured as percentage of yield conversion.RESULTS: The optimal conditions obtained were: temperature, 31.5 °C; reaction time, 7 h; substrate molar ratio, 7.5:1 ethanol:soybean oil; enzyme content, 15% (g enzyme g⁻¹ oil); added water, 4% (g water g⁻¹ oil). The experimental yield conversion obtained under these conditions was 96%, which is very close to the maximum predicted value of 94.4%. The reaction time-course at the optimal values indicated that 5 h was necessary to obtain high yield conversions.CONCLUSION: A high yield conversion was obtained under the optimized conditions, with relative low enzyme content and short time. Comparison of predicted and experimental values showed good correspondence, implying that the empirical model derived from RSM can be used to adequately describe the relationship between the reaction parameters and the response (yield conversion) in lipase-catalyzed biodiesel synthesis. Copyright © 2008 Society of Chemical Industry
ISSN:0268-2575
1097-4660
DOI:10.1002/jctb.1879