Comparative study of poly(vinyl alcohol)-based support materials for the immobilization of glucose oxidase

BACKGROUND: The performances of four types of glucose oxidase (GOD) immobilization materials based on poly(vinyl alcohol) (PVA) were compared. The matrices of interest were chemically‐linked PVA, freeze‐thawed PVA cryogel, tetramethoxysilane (TMOS) sol‐gel‐PVA hybrid material, and alumina sol‐gel‐PV...

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Bibliographic Details
Published in:Journal of chemical technology and biotechnology (1986) 2008-01, Vol.83 (1), p.41-46
Main Authors: Wong, Fui-Ling, Abdul-Aziz, Azila
Format: Article
Language:English
Subjects:
Applied sciences
Chemical engineering
Exact sciences and technology
glucose oxidase
hybrid
immobilization
poly(vinyl alcohol)
sol-gel
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Summary:BACKGROUND: The performances of four types of glucose oxidase (GOD) immobilization materials based on poly(vinyl alcohol) (PVA) were compared. The matrices of interest were chemically‐linked PVA, freeze‐thawed PVA cryogel, tetramethoxysilane (TMOS) sol‐gel‐PVA hybrid material, and alumina sol‐gel‐PVA hybrid material. RESULTS: Overall, the membranes showed good sensitivity except for the chemically cross‐linked PVA. However, the main differences with the enzyme immobilization methods were enzyme leakage and values of Kmapp. CONCLUSION: Freeze‐thawed PVA‐GOD membranes and TMOS‐PVA, which showed satisfactory sensitivity and adequate value of Kmapp, were quite promising as support materials for immobilizing GOD. Copyright © 2007 Society of Chemical Industry
ISSN:0268-2575
1097-4660
DOI:10.1002/jctb.1774