Comparing Baseline VAF in Circulating tumor DNA and Tumor Tissues Predicting Prognosis of Patients with Colorectal Cancer Liver Metastases After Curative Resection

•NGS of paired baseline serum and tumor tissues of colorectal liver metastases patients could provide prognostic value for them after curative resection.•Baseline VAF in ctDNA but not in tumor tissues influenced RFS of colorectal liver metastases patients after curative resection.•Neither baseline V...

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Published in:Clinics and research in hepatology and gastroenterology 2024-09, p.102464, Article 102464
Main Authors: Jin, Ke-min, bao, Quan, Zhao, Ting-ting, Wang, Hong-wei, Huang, Long-fei, Wang, Kun, Xing, Bao-cai
Format: Article
Language:English
Subjects:
baseline ctDNA
colorectal liver metastases
next-generation sequencing
prognosis
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Summary:•NGS of paired baseline serum and tumor tissues of colorectal liver metastases patients could provide prognostic value for them after curative resection.•Baseline VAF in ctDNA but not in tumor tissues influenced RFS of colorectal liver metastases patients after curative resection.•Neither baseline VAF in ctDNA nor in tumor tissues influenced OS of colorectal liver metastases patients after curative resection. The prognostic value of baseline variant allele frequency (VAF) in circulating tumor DNA (ctDNA) of colorectal cancer liver metastases (CRLM) patients after curative resection was rarely investigated. A single-center prospective study was performed to investigate the prognostic impact of baseline VAF in ctDNA and matched tumor tissues of CRLM patients after curative resection between May 2019 and May 2021 by the Illumina NovoSeq 6000 platform. The relationship of the tumor burden score (TBS) and the VAF in ctDNA and matched tumor tissues was evaluated by the Pearson correlation method. The survival curves of recurrence-free survival (RFS) and overall survival (OS) were plotted. Factors associated with RFS were calculated using Cox regression analysis, and an integrated prognostic model using significant baseline variables was proposed. There were 121 patients with baseline ctDNA and matched tumor tissues enrolled in the study. A total of 417 mutations spanning 20 genes were identified in baseline tumor tissues of 119/121 (98.3%) cases. The overall mutations in tumor tissues were completely covered by ctDNA in 52 of 121(43.0%) patients. Baseline VAF in ctDNA but not in tumor tissues was significantly correlated to TBS of CRLM (R=0.36, p
ISSN:2210-7401
2210-741X
2210-741X
DOI:10.1016/j.clinre.2024.102464