Flubendiamide provokes oxidative stress, inflammation, miRNAs alteration, and cell cycle deregulation in human prostate epithelial cells: The attenuation impact of synthesized nano-selenium using Trichodermaaureoviride

Flubendiamide (FBD) is a novel diamide insecticide extensively used with potential human health hazards. This research aimed to examine the effects of FBD on PrEC prostate epithelial cells, including Oxidative stress, pro-inflammatory responses, modifications in the expression of oncogenic and suppr...

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Bibliographic Details
Published in:Chemosphere (Oxford) 2024-10, Vol.365, p.143305, Article 143305
Main Authors: Arafa, Samah S., Badr El-Din, Sahar, Hewedy, Omar A., Abdelsattar, Shimaa, Hamam, Sanaa S., Sharif, Asmaa F., Elkholy, Reem Mohsen, Shebl, Ghada Zaghloul, Al-Zahrani, Majid, Salama, Rasha Aziz Attia, Abdelkader, Afaf
Format: Article
Language:English
Subjects:
Flubendiamide
Nano-selenium particles
Prostate cells
Trichoderma aureoviride
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Summary:Flubendiamide (FBD) is a novel diamide insecticide extensively used with potential human health hazards. This research aimed to examine the effects of FBD on PrEC prostate epithelial cells, including Oxidative stress, pro-inflammatory responses, modifications in the expression of oncogenic and suppressor miRNAs and their target proteins, disruption of the cell cycle, and apoptosis. Additionally, the research investigated the potential alleviative effect of T-SeNPs, which are selenium nanoparticles biosynthesized by Trichoderma aureoviride, against the toxicity induced by FBD. Selenium nanoparticles were herein synthesized by Trichoderma aureoviride. The major capping metabolites in synthesized T-SeNPs were Isochiapin B and Quercetin 7,3′,4′-trimethyl ether. T-SeNPs showed a spherical shape and an average size between 57 and 96.6 nm. FBD exposure (12 μM) for 14 days induced oxidative stress and inflammatory responses via overexpression of NF-κB family members. It also distinctly caused upregulation of miR-221, miR-222, and E2F2, escorted by downregulation of miR-17, miR-20a, and P27kip1. FBD encouraged PrEC cells to halt at the G1/S checkpoint. Apoptotic cells were drastically increased in FBD-treated sets. Treatment of T-SeNPs simultaneously with FBD revealed its antioxidant, anti-inflammatory, and antitumor activities in counteracting FBD-induced toxicity. Our findings shed light on the potential FBD toxicity that may account for the neoplastic transformation of epithelial cells in the prostate and the mitigating activity of eco-friendly synthesized T-SeNPs. [Display omitted] •FBD promoted oxidative stress and pro-inflammatory response.•FBD caused upregulation of miR-221/222 and downregulation of miR-17/20a.•FBD altered the protein level of E2F2 and P27kip1.•FBD caused cell cycle arrest at the G1/S checkpoint and apoptosis.•T-SeNPs rescued the insecticide's adverse effects.
ISSN:0045-6535
1879-1298
1879-1298
DOI:10.1016/j.chemosphere.2024.143305