Isolation of lipase producing Bacillus sp. from olive mill wastewater and improving its enzyme activity

The bacteria that could grow on media containing olive mill wastewater (OMW) were isolated and their lipase production capacities were investigated. The strain possessing the highest lipase activity among 17 strains grown on tributyrin agar medium was identified as Bacillus sp. The effect of initial...

Full description

Saved in:
Bibliographic Details
Published in:Journal of hazardous materials 2007-11, Vol.149 (3), p.720-724
Main Authors: Ertugrul, Sevgi, Donmez, Gonul, Takac, Serpil
Format: Article
Language:English
Subjects:
Agar - chemistry
Applied sciences
Bacillus - enzymology
Bacillus - metabolism
Bacillus sp
Biological and medical sciences
Culture Media - chemistry
Enzymes - chemistry
Exact sciences and technology
Fatty Acids - chemistry
Food engineering
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
General purification processes
Hydrogen-Ion Concentration
Industrial Microbiology - methods
Industrial Waste
Isolation
Lipase
Lipase - chemistry
Lipolysis
Olea
Olea - chemistry
Olive mill wastewater
Pollution
Temperature
Time Factors
Triglycerides - chemistry
Waste Disposal, Fluid - methods
Wastewaters
Water treatment and pollution
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The bacteria that could grow on media containing olive mill wastewater (OMW) were isolated and their lipase production capacities were investigated. The strain possessing the highest lipase activity among 17 strains grown on tributyrin agar medium was identified as Bacillus sp. The effect of initial pH on the lipase activity was investigated in tributyrin medium and pH 6 was found to be the optimal. The liquid medium composition was improved by replacing tributyrin with various carbon sources. Among the media containing different compositions of triolein, trimyristin, trilaurin, tricaprin, tricaprylin, tributyrin, triacetin, Tween 80, OMW, glucose, and whey; the medium contained 20% whey +1% triolein was found to give the highest lipase activity. Cultivation of Bacillus sp. in the optimal medium at pH 6 and 30 °C for 64 h resulted in the extracellular and intracellular lipase activities of 15 and 168 U/ml, respectively.
ISSN:0304-3894
1873-3336
DOI:10.1016/j.jhazmat.2007.04.034