A salt/salt aqueous two-phase system based on pH-switchable deep eutectic solvent for the extraction and separation of mulberry polysaccharides

With the aim of expanding the potential application scope of mulberries, eleven pH-switchable deep eutectic solvents were screened for the ultrasonic-assisted extraction of mulberry polysaccharides, and a salt/salt aqueous two-phase system was constructed for the efficient separation of mulberry pol...

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Bibliographic Details
Published in:Food chemistry 2025-01, Vol.462, p.141024, Article 141024
Main Authors: Lei, Tian, Qin, Zongkui, Liu, Leilei, Tan, Zhijian
Format: Article
Language:English
Subjects:
Aqueous two-phase systems
Mulberry
Polysaccharides
Separation and purification
Switchable deep eutectic solvents
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Summary:With the aim of expanding the potential application scope of mulberries, eleven pH-switchable deep eutectic solvents were screened for the ultrasonic-assisted extraction of mulberry polysaccharides, and a salt/salt aqueous two-phase system was constructed for the efficient separation of mulberry polysaccharides by regulating the system pH. DES-9 (tetraethylammonium chloride: octanoic acid molar ratio = 1: 2) with a critical response pH value of approximately 6.1 was concluded to be the best extraction solvent for extracting mulberry polysaccharides. A maximum polysaccharide extraction yield of 270.71 mg/g was obtained under the optimal conditions. The maximum polysaccharide extraction efficiency was 78.09 % for the pH-driven tetraethylammonium chloride/K2HPO4 aqueous two-phase system. An acidic β-pyran mulberry polysaccharide with a low-molecular weight of 9.26 kDa and a confirmed monosaccharide composition were obtained. This efficient and environmentally friendly polysaccharide separation method offers a new approach for the efficient extraction and utilization of other plant polysaccharides. •pH-switchable deep eutectic solvent was used for mulberry polysaccharides extraction.•This salt/salt aqueous two-phase system exhibited high extraction efficiency.•An acidic β-pyran polysaccharide with a molecular weight of 9.26 kDa was obtained.
ISSN:0308-8146
1873-7072
1873-7072
DOI:10.1016/j.foodchem.2024.141024