Assessing reproductive effects and epigenetic responses in Austrolebias charrua exposed to Roundup Transorb®: Insights from miRNA profiling and molecular interaction analysis

This study examines the effects of Roundup Transorb® (RDT) exposure on reproductive functions and ovarian miRNA expression in Austrolebias charrua. Exposure to RDT (at 0.065 or 5 mg. L−1 for 96 h) significantly disrupts fertility, evidenced by changes in fertilization rates and egg diameter. Profili...

Full description

Saved in:
Bibliographic Details
Published in:Environmental toxicology and pharmacology 2024-09, Vol.110, p.104539, Article 104539
Main Authors: Pagano, Antônio D., Nunes, Leandro S., Domingues, William B., da Silveira, Tony L.R., Kütter, Mateus T., Schneider, Augusto, Kremer, Frederico S., Junior, Antonio S.V., Amaral, Marta G., Gonçalves, Natiéli M., Bellido-Quispe, Dionet K., Volcan, Matheus V., Costa, Patrícia G., Bianchini, Adalto, Pinhal, Danillo, Campos, Vinicius F., Remião, Mariana H.
Format: Article
Language:English
Subjects:
Biodiversity
Ecotoxicogenomics
Glyphosate
Interactome analysis
MicroRNA sequencing
Reproductive toxicology
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study examines the effects of Roundup Transorb® (RDT) exposure on reproductive functions and ovarian miRNA expression in Austrolebias charrua. Exposure to RDT (at 0.065 or 5 mg. L−1 for 96 h) significantly disrupts fertility, evidenced by changes in fertilization rates and egg diameter. Profiling of ovarian miRNAs identified a total 205 miRNAs in A. charrua. Among these, three miRNAs were upregulated (miR-10b-5p, miR-132–3p, miR-100–5p), while ten miRNAs were downregulated (miR-499–5p, miR-375, miR-205–5p, miR-206–3p, miR-203a-3p, miR-133b-3p, miR-203b-5p, miR-184, miR-133a-3p, miR-2188–5p) compared to non-exposed fish. This study reveals that differentially expressed miRNAs are linked to molecular pathways such as steroid hormone biosynthesis, lipid and carbohydrate metabolism, bioenergetics, and antioxidant defense. It also analyzes molecular interactions between miRNAs and target genes during RDT exposure in annual killifish, providing insights into biomarkers in ecotoxicology. Moreover, it provides scope for developing environmental health assessment models based on epigenomic endpoints, supporting the protection of biodiversity and ecosystem services through the quantification of stress responses in living organisms exposed to pesticides. •Roundup Transorb® exposure disrupts fertility in the endangered annual killifish A. charrua.•205 mature miRNAs were identified in A. charrua ovarian tissue.•13 ovarian miRNAs were revealed as potential biomarkers for Roundup Transorb® exposure.•The DEmiRNAs control steroidogenesis, lipogenesis, and stress responses pathways.•miRNAs are useful biomarkers for the early detection of chemical exposure in fish.
ISSN:1382-6689
1872-7077
1872-7077
DOI:10.1016/j.etap.2024.104539