Amphoteric hydrogels for immobilization of enzymes using template polymerization technique

Novel ionizable amphoteric hydrogels were prepared from poly(acrylic acid) and dimethylaminoethyl methacrylate monomer, employing template polymerization technique. The mode of interaction, as proved by FTIR, was multiple H-bonding between the tertiary amino group of the monomer and the carboxylic g...

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Bibliographic Details
Published in:Journal of applied polymer science 2007-12, Vol.106 (6), p.3571-3580
Main Authors: El-Masry, Mansour M, Elnashar, Magdy M.M, El-Sherif, Hazem M
Format: Article
Language:English
Subjects:
amphoteric hydrogels
Applied sciences
Biological and medical sciences
Biotechnology
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Immobilization of enzymes and other molecules
Immobilization techniques
Methods. Procedures. Technologies
non-Fickian mechanism
Organic polymers
Physicochemistry of polymers
polyacrylic acid
Properties and characterization
Solution and gel properties
template polymerization
β-galactosidase immobilization
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Summary:Novel ionizable amphoteric hydrogels were prepared from poly(acrylic acid) and dimethylaminoethyl methacrylate monomer, employing template polymerization technique. The mode of interaction, as proved by FTIR, was multiple H-bonding between the tertiary amino group of the monomer and the carboxylic groups of the polymer. The impact of varying equal polymer-monomer feed ratios from 0.1 to 1.1 on the swelling dynamics was examined. Penetrant sorption experiments demonstrated that the swelling behavior depends strongly on the polymer complex composition. The polymer complex of feed ratio 0.5 : 0.5 (polymer : monomer) showed maximum swelling percentage. The mechanism of the polymer complexes swelling was probably a non-Fickian with n values approaching Fickian behavior. The hydrogels showed maximum swelling efficiencies of 27 folds and 13.5 folds in drastic acidic and basic medium, respectively, using polymer complex of 0.5 : 0.5 feed ratio. Because of reversibility and rapidity of swelling, the gel could be considered as a mechanochemical system. The prepared hydrogel successfully immobilized the industrially used β-galactosidase as an acidic model enzyme. The novel immobilized enzyme showed a remarkable improvement in its activity (13.8 μmol min⁻¹ mg⁻¹) compared to the free enzyme (3.2 μmol min⁻¹ mg⁻¹). The optimum pH values for free and immobilized enzyme were 4.5-5 and 4, respectively. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci, 2007
ISSN:0021-8995
1097-4628
DOI:10.1002/app.26931