Efficient and fast detection of uric acid based on a colorimetric sensing method

The uric acid (UA) level is an important physiological indicator of the human body, and its abnormality can lead to a series of diseases. Therefore, the immediate detection of uric acid concentration has broad application prospects. Commonly used methods for the analysis of uric acid include chromat...

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Bibliographic Details
Published in:Analytical methods 2024-08, Vol.16 (34), p.5896-5901
Main Authors: Bai, Yunfeng, Fan, Xiaoxuan, Chen, Guoning, Zhao, Zhilong
Format: Article
Language:English
Subjects:
Bioassays
Capillary electrophoresis
Colorimetry
Cost analysis
Electrochemistry
Electrophoresis
Hydrogen peroxide
Oxidation
Uric acid
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Summary:The uric acid (UA) level is an important physiological indicator of the human body, and its abnormality can lead to a series of diseases. Therefore, the immediate detection of uric acid concentration has broad application prospects. Commonly used methods for the analysis of uric acid include chromatography, high-performance capillary electrophoresis and electrochemical methods. However, these methods have the disadvantages of cumbersome sample pre-treatment, high cost, time-consuming, and the need for experimental instruments and professional operators, which are extremely unfavorable for the detection of uric acid and the diagnosis of related diseases in resource-limited areas. In this study, a portable visualization method was developed for the detection of uric acid using hydrogen peroxide (H 2 O 2 ) test strips. Uric acid enzyme specifically catalyzes the oxidation of uric acid to produce H 2 O 2 , which causes a significant change in the color of the H 2 O 2 test strip. The response has good linearity in the range of 1 ∼ 50 μg mL −1 . Thus, it provides a simple, rapid, and cost-effective visualized bioassay for uric acid.
ISSN:1759-9660
1759-9679
1759-9679
DOI:10.1039/d4ay01053a