Loading…
RP-HPLC-RID analysis of InsP6 to InsP3 in Indian cereals, legumes, and their products: A comparative evaluation of PRP-1 Vs C18 column
•RP-HPLC-RID method is simple and robust for quantification of IPs in food.•Methanol: 0.035 M Formic acid (58:42) + 0.7 ml/100 ml TBAOH gave best resolution.•C-18 column gave best accuracy & precision for simultaneous determination of InsPs.•This method enables cost effective and high sample thr...
Saved in:
| Published in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2024-09, Vol.1245, p.124271, Article 124271 |
|---|---|
| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
| Citations: | Items that this one cites |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | •RP-HPLC-RID method is simple and robust for quantification of IPs in food.•Methanol: 0.035 M Formic acid (58:42) + 0.7 ml/100 ml TBAOH gave best resolution.•C-18 column gave best accuracy & precision for simultaneous determination of InsPs.•This method enables cost effective and high sample throughput sample analysis.•Processed foods contained lesser InsP6 and more of dephosphorylated InsP6.•Wheat products completely lacked lower InsPs.
Phytic acid or inositol hexakisphosphate (InsP6) and its dephosphorylated forms (InsP5, InsP4 & InsP3) are integral to cellular functions and confer several health benefits. The present study was aimed to develop a cost effective and high sample throughput RP-HPLC-RID method for routine quantification of lower inositol phosphates in both raw and processed cereals and pulses. For this asuitable mobile phase composition was formulated and two columns (MacroporusHamilton PRP-1 Vs Waters Symmetry C18) were compared in terms ofsystem specificity,linearity, accuracy and precision. Separation ofInsP3, InsP4, InsP5 and InsP6 were recorded at 2.39, 2.93, 3.83 and 5.37 min usingPRP-1column while the RT were 4.67, 5.64, 6.99 and 9.14 min with C18column.Linearity of standards (R2 > 0.99), with an accuracy and precision ranging from 1 to 5 % was achieved. The LOD and LOQ of all InsPs were 5 and 15 μg/ml, respectively. In quality control sample InsP6 was found in highest concentration (446 ± 14.71 mg/100 g) followed by InsP5 (162 ± 8.00 mg/100 g) and InsP4 with the least concentration of 11.63 ± 1.06 mg/100 g whereas InsP3 was below detectable limit (BDL). The optimised method was used for profiling of InsPs in the raw and processed cereals and pulses consumed as staple foods in India. Processed foods contained lesser InsP6 and more of lower InsP compared to raw foods. The optimised method using unique mobile phase composition was found to yield accurate results and can used for large scale analysis of cereals and pulses and estimation of mineral nutrition potential and allied health benefits. |
|---|---|
| ISSN: | 1570-0232 1873-376X 1873-376X |
| DOI: | 10.1016/j.jchromb.2024.124271 |