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Synergistic berberine chloride and Curcumin-Loaded nanofiber therapies against Methicillin-Resistant Staphylococcus aureus Infection: Augmented immune and inflammatory responses in zebrafish wound healing
[Display omitted] •The optimized concentration of curcumin and berberine chloride yields Cu@Bbc nanofiber, demonstrating potent antibacterial effects against MRSA and sustained drug release.In vitro analysis reveals enhanced wound healing potential of Cu@Bbc nanofiber through increased cell migratio...
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| Published in: | International immunopharmacology 2024-10, Vol.140, p.112856, Article 112856 |
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| Main Authors: | , , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites |
| Online Access: | Get full text |
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| Summary: | [Display omitted]
•The optimized concentration of curcumin and berberine chloride yields Cu@Bbc nanofiber, demonstrating potent antibacterial effects against MRSA and sustained drug release.In vitro analysis reveals enhanced wound healing potential of Cu@Bbc nanofiber through increased cell migration in Fibroblast cells.•In vivo results in zebrafish support therapeutic promise, accelerating wound closure and tissue remodeling processes, enhancing overall wound efficacy.•The inflammatory response of wounded tissue in zebrafish was meticulously analyzed, revealing well-defined structural tissue regeneration dynamics.•Treatment with Cu@Bbc nanofibers demonstrated a robust adaptive immune response, as evidenced by the formation of an epithelial layer accompanied by immune cell infiltration, thus reinforcing the histological findings.
Wound healing pivots on a finely orchestrated inflammatory cascade, critical for tissue repair. Chronic wounds, compounded by persistent inflammation and susceptibility to infection, pose formidable clinical challenges. Nanofiber dressings offer promising avenues for wound care, yet their interaction with inflammation and infection remains elusive. We aim to delineate the inflammatory cascade preceding wound closure and assess Cu@Bbc nanofibers’ therapeutic efficacy in mitigating inflammation and combating infection. Their unique attributes suggest promise in modulating inflammation, fostering tissue regeneration, and preventing microbial colonization. Investigating the intricate interplay between nanofiber scaffolds, inflammation, and infection may unveil mechanisms of enhanced wound healing. Our findings could stimulate the development of tailored dressings, urgently needed for effective wound management amidst immune dysregulation, infection, and inflammation.
In this investigation, we synthesized Cu@Bbc nanofibers, incorporating curcumin and berberine chloride, for wound healing applications. We evaluated their individual and combined antibacterial, anti-biofilm, and antioxidant activities, alongside binding affinity with pro-inflammatory cytokines through molecular docking. Morphological characterization was conducted via SEM, FTIR assessed functional groups, and wettability contact angle measured hydrophobic properties. The physical properties, including tensile strength, swelling behavior, and thermal stability, were evaluated using tensile testing, saline immersion method and thermogravimetric analysis. Biodegradability of |
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| ISSN: | 1567-5769 1878-1705 1878-1705 |
| DOI: | 10.1016/j.intimp.2024.112856 |