Novel glucomannan-like polysaccharide from Lycium barbarum L. ameliorates renal fibrosis via blocking macrophage-to-myofibroblasts transition

The macrophage to myofibroblasts transition (MMT) has been reported as a newly key target in renal fibrosis. Lycium barbarum L. is a traditional Chinese medicine for improving renal function, in which its polysaccharides (LBPs) are the mainly active components. However, whether the role of LBPs in t...

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Published in:International journal of biological macromolecules 2024-10, Vol.278 (Pt 2), p.134491, Article 134491
Main Authors: Liu, Tian, Zhang, Yun, Wu, Ze, Zhao, Chen-jing, Dong, Xi, Gong, He-xin, Jin, Bing, Han, Miao-miao, Wu, Jin-jia, Fan, Yi-kai, Li, Nan, Xiong, Ying-xia, Zhang, Zi-qian, Dong, Zheng-qi
Format: Article
Language:English
Subjects:
Chemical structure
Lycium barbarum L
Macrophage to myofibroblasts transition
Polysaccharide
Renal fibrosis
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Summary:The macrophage to myofibroblasts transition (MMT) has been reported as a newly key target in renal fibrosis. Lycium barbarum L. is a traditional Chinese medicine for improving renal function, in which its polysaccharides (LBPs) are the mainly active components. However, whether the role of LBPs in treating renal fibrosis is related to MMT process remain unclear. The purpose of this study was to explore the relationship between the regulating effect on MMT process and the anti-fibrotic effect of LBPs. Initially, small molecular weight LBPs fractions (LBP-S) were firstly isolated via Sephadex G-100 column. Then, the potent inhibitory effect of LBP-S on MMT process was revealed on bone marrow-derived macrophages (BMDM) model induced by TGF-β. Subsequently, the chemical structure of LBP-S was elucidated through monosaccharide, methylation and NMR spectrum analysis. In vivo biodistribution characteristics studies demonstrated that LBP-S exhibited effectively accumulation in kidney via intraperitoneal administration. Finally, LBP-S showed a satisfactory anti-renal fibrotic effect on unilateral ureteral obstruction operation (UUO) mice, which was significantly reduced following macrophage depletion. Overall, our findings indicated that LPB-S could alleviate renal fibrosis through regulating MMT process and providing new candidate agents for chronic kidney disease (CKD) related fibrosis treatment. Diagram showing the extraction and isolation, structural characterization and MMT effectiveness of Lycium barbarum L. polysaccharides. [Display omitted]
ISSN:0141-8130
1879-0003
1879-0003
DOI:10.1016/j.ijbiomac.2024.134491