Do exchangeable hydrogens affect the evaluation of partial mycoheterotrophy in orchids? Insights from δ2H analysis in bulk, α‐cellulose, and cellulose nitrate samples

Summary To evaluate the nutritional modes of orchids associated with ‘rhizoctonia’ fungi, analyses of hydrogen (δ2H), carbon (δ13C), and nitrogen (δ15N) stable isotope ratios are usually adopted. However, previous studies have not fully accounted for exchangeable hydrogens, which could affect these...

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Bibliographic Details
Published in:The New phytologist 2024-09, Vol.243 (6), p.2430-2441
Main Authors: Yagi, Ryuta, Haraguchi, Takashi F., Tayasu, Ichiro, Suetsugu, Kenji
Format: Article
Language:English
Subjects:
Autotrophs
Bulk sampling
Calanthe
Carbon 13
Cellulose
Cellulose nitrate
exchangeable hydrogen
Fungi
Gastrodia
Heterogeneity
Impact analysis
Isotope ratios
Modes
mycoheterotrophy
mycorrhizal symbiosis
Nutrition assessment
Orchidaceae
Plant species
rhizoctonia
Stable isotopes
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Summary:Summary To evaluate the nutritional modes of orchids associated with ‘rhizoctonia’ fungi, analyses of hydrogen (δ2H), carbon (δ13C), and nitrogen (δ15N) stable isotope ratios are usually adopted. However, previous studies have not fully accounted for exchangeable hydrogens, which could affect these evaluations. Here, we performed standard δ13C, δ15N, and δ2H analyses on bulk samples. Additionally, we conducted δ2H analysis on α‐cellulose and cellulose nitrate samples to investigate whether the heterogeneity of exchangeable hydrogens among plant species influences the assessment of nutritional modes. The δ2H of orchids were consistently higher than those of surrounding autotrophic plants, irrespective of the three pretreatments. Although the rhizoctonia‐associated orchid exhibited lower δ13C, its δ2H was higher than those of the autotrophs. Notably, among all response variables, δ15N and δ2H exhibited high abilities for discriminating the nutritional modes of rhizoctonia‐associated orchids. These results indicate that a time‐efficient bulk sample analysis is an effective method for evaluating plant nutritional modes, as the heterogeneity of exchangeable hydrogens does not significantly impact the estimation. Using δ15N and δ2H benefits the assessment of partial mycoheterotrophy among rhizoctonia‐associated orchids.
ISSN:0028-646X
1469-8137
1469-8137
DOI:10.1111/nph.19998