Genome editing tools based improved applications in macrofungi

Macrofungi commonly referred to as Mushrooms are distributed worldwide and well known for their nutritional, medicinal, and organoleptic properties. Strain improvement in mushrooms is lagging due to paucity of efficient genome modification techniques. Thus, for advanced developments in research and...

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Bibliographic Details
Published in:Molecular biology reports 2024-12, Vol.51 (1), p.873, Article 873
Main Authors: Jain, Deepali, Kalia, Anu, Sharma, Shivani, Manchanda, Pooja
Format: Article
Language:English
Subjects:
Animal Anatomy
Animal Biochemistry
Biomedical and Life Sciences
CRISPR
Fruit bodies
Fungiculture
Genome editing
Genomes
Histology
Hybridization
Life Sciences
Mini Review
Morphology
Mushrooms
Organoleptic properties
Protoplast fusion
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Summary:Macrofungi commonly referred to as Mushrooms are distributed worldwide and well known for their nutritional, medicinal, and organoleptic properties. Strain improvement in mushrooms is lagging due to paucity of efficient genome modification techniques. Thus, for advanced developments in research and commercial or economical viability and benefit, CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease 9) emerged as an efficient genome editing tool. The higher efficiency and precision of the desired genetic modification(s) are the most valuable attributes of this recent technology. The present review comprehensively summarizes various conventional methods utilized for strain improvement in mushrooms including hybridization, protoplast fusion, and di-mon mating. Furthermore, the problems associated with these techniques have been discussed besides providing the potential recluses. The significance of CRISPR/Cas9 strategies employed for improvement in various mushroom genera has been deliberated, as these strategies will paves the way forward for obtaining improved strain and effective cultivation methods for enhancing the yield and quality of the fruit bodies.
ISSN:0301-4851
1573-4978
1573-4978
DOI:10.1007/s11033-024-09809-7