Region‐specific gene expression profile in the epididymis of high‐ and low‐fertile dromedary camels

The scenario of the fertile spermatozoa with high fertilizing capability is basically dependent on gene expression‐based epididymal function. The current investigation aimed to declare the varied expression of different candidate genes (PLA2G4D, LCN15, CLUAP1, SPP1, AQP12B, DEFB110 and ESR1) relevan...

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Bibliographic Details
Published in:Reproduction in domestic animals 2024-07, Vol.59 (7), p.e14678-n/a
Main Authors: Rashad, Dina E. M., Ibrahim, Sally, El‐Sokary, Mohamed M. M., Mahmoud, Karima Gh. M., Kandiel, Mohamed M. M., Abou El‐Roos, Mahmoud E. A., Sosa, Gamal A. M.
Format: Article
Language:English
Subjects:
Animals
Breeding seasons
Camelidae
Camelids
Camels
Camelus - genetics
dromedary camel
Epididymis
Epididymis - metabolism
epididymis regions
ESR1 protein
Fertility
Fertility - genetics
Gene expression
Genes
Male
Morphology
Physical characteristics
Segments
Sperm
Sperm Motility
Spermatozoa
Spermatozoa - metabolism
Transcriptome
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Summary:The scenario of the fertile spermatozoa with high fertilizing capability is basically dependent on gene expression‐based epididymal function. The current investigation aimed to declare the varied expression of different candidate genes (PLA2G4D, LCN15, CLUAP1, SPP1, AQP12B, DEFB110 and ESR1) relevant to spermatozoa features between the different epididymal segments in the mature dromedary camels (n = 30). Scrotal contents were collected post‐slaughtering, during the breeding season and the epididymis was separated from the testicles and divided into three segments (caput, corpus and cauda) based on its morphology and anatomical characteristics. Epididymal spermatozoa were harvested from each epididymal portion and evaluated for motility, count, viability and morphology. Samples were grouped depending on their epididymal sperm cells features into high‐fertile (n = 15) and low‐fertile (n = 15) groups. The gene expression of the candidate genes was defined in the isolated RNA from each epididymal portion tissue. The segmental sperm motion and count were significantly (p 
ISSN:0936-6768
1439-0531
1439-0531
DOI:10.1111/rda.14678