In vitro, in vivo and in silico antiplasmodial profiling of the aqueous extract of Hibiscus asper HOOK F. Leaf (Malvaceae)

Plasmodium resistance to antimalarial drugs raises the urgent need to seek for alternative treatments. Aqueous extract of Hibiscus asper leaves is currently used in malaria management but remains less documented. Aim of the study: The study aims to evaluate antimalarial effects of the aqueous extrac...

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Published in:Journal of ethnopharmacology 2024-12, Vol.335, p.118536, Article 118536
Main Authors: Tsakem Nangap, Marius Jaurès, Walbadet, Lucain, Mbock, Michel Arnaud, Adjieufack, Abel Idrice, Ongagna, Jean Moto, Fokou, Roberto, Tenlep, Loïc Ngwem, Tchatat, Mariscal Brice, Tsouh Fokou, Patrick Valère, Boyom, Fabrice Fekam, Gounoue kamkumo, Raceline, Tsofack, Florence Ngueguim, Dimo, Théophile
Format: Article
Language:English
Subjects:
Antimalarial effects
Antioxidant properties
Hibiscus asper
Molecular docking
Plasmodium
UHPLC-MS analysis
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Summary:Plasmodium resistance to antimalarial drugs raises the urgent need to seek for alternative treatments. Aqueous extract of Hibiscus asper leaves is currently used in malaria management but remains less documented. Aim of the study: The study aims to evaluate antimalarial effects of the aqueous extract of Hibiscus asper. UHPLC/MS, was used to identify some likely compounds present in the plant that were thereafter docked to some malaria parasite proteins. In vitro anti-plasmodium and antioxidant, UHPLC/Ms analysis, in vivo antimalarial of the plant extract, and in silico molecular docking prediction of some identified compounds were performed to investigate the pharmacological effects of H. asper. The in vitro antiplasmodial activity of the extract was carried out on Plasmodium falciparum strains using SYBR-green dye; then, the curative antimalarial activity was conducted on Plasmodium berghei NK65-infected male Wistar rats. The UHPLC/MS analysis was used to identify plant compounds, followed by interactions (docking affinity) between some compounds and parasitic enzymes such as P. falciparum purine nucleoside phosphorylase (2BSX) and 6-phosphogluconate dehydrogenase (6FQY) to explore potential mechanisms of action at the molecular level. No hemolysis effect of the extract was observed at concentrations up to 100 mg/mL. In vitro test of the aqueous leaves extract of H. asper showed inhibitory activity against P. falciparum Dd2 and 3D7 strains with IC50 values of 19.75 and 21.97 μg/mL, respectively. The curative antimalarial test of the H. asper extract in infected rats exhibited significant inhibition of the parasite growth (p 
ISSN:0378-8741
1872-7573
1872-7573
DOI:10.1016/j.jep.2024.118536