Multi-Shell Nanourchin-Integrated Dual Mode Lateral Flow Immunoassay for Sensitive and Rapid Detection of Clinical Cardiac Myosin-Binding Protein C

Cardiac myosin-binding protein C (cMyBP-C) is a novel cardiac marker of acute myocardial infarction (AMI) and acute cardiac injuries (ACI). Construction of point-of-care testing techniques capable of sensing cMyBP-C with high sensitivity and precision is urgently needed. Herein, we synthesized an Au...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2024-07, Vol.96 (29), p.11853-11861
Main Authors: Xu, Xuan, Yue, Shuzhen, Tu, Keke, Yuan, Baozhen, Bi, Sai, Yu, Jinjin, Qiu, Hui, Zhang, Haotian, Zhang, Lei, Wu, Heng-Fang, Chen, Xiang-Jian, Zhao, Sheng, Zhang, Wei, Zhang, Ji-Nan, Jiang, Li-Ping, Zhang, Jian-Rong, Zhu, Jun-Jie
Format: Article
Language:English
Subjects:
Color
Colorimetry
Heart
Immunoassay
Myocardial infarction
Myosin
Myosin-binding protein C
Protein C
Proteins
Sensitivity analysis
Smartphones
Strip
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Summary:Cardiac myosin-binding protein C (cMyBP-C) is a novel cardiac marker of acute myocardial infarction (AMI) and acute cardiac injuries (ACI). Construction of point-of-care testing techniques capable of sensing cMyBP-C with high sensitivity and precision is urgently needed. Herein, we synthesized an Au@NGQDs@Au/Ag multi-shell nanoUrchins (MSNUs), and then applied it in a colorimetric/SERS dual-mode immunoassay for detection of cMyBP-C. The MSNUs displayed superior stability, colorimetric brightness, and SERS enhancement ability with an enhanced factor of 5.4 × 109, which were beneficial to improve the detection capability of test strips. The developed MSNU-based test strips can achieve an ultrasensitive immunochromatographic assay of cMyBP-C in both colorimetric and SERS modes with the limits of detection as low as 19.3 and 0.77 pg/mL, respectively. Strikingly, this strip was successfully applied to analyze actual plasma samples with significantly better sensitivity, negative predictive value, and accuracy than commercially available gold test strips. Notably, this method possessed a wide range of application scenarios via combining with a color recognizer application named Color Grab on the smartphone, which can meet various needs of different users. Overall, our MSNU-based test strip as a mobile health monitoring tool shows excellent sensitivity, reproducibility, and rapid detection of the cMyBP-C, which holds great potential for the early clinic diagnosis of AMI and ACI.
ISSN:0003-2700
1520-6882
1520-6882
DOI:10.1021/acs.analchem.4c01514