Genome-wide identification, characterization and expression profiling of TLR family genes in Chromileptesaltivelis

Toll-like receptors (TLRs) represent a prominent category of pattern recognition receptors that have been extensively investigated for their pivotal role in combating pathogen incursions. Despite this, there has been a notable absence of comprehensive identification and exploration of the immune res...

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Bibliographic Details
Published in:Fish & shellfish immunology 2024-08, Vol.151, p.109720, Article 109720
Main Authors: Ren, Yanjie, Kong, Miao, Sun, Huibang, Zhao, Benqi, Wu, Hanwei, Chen, Zhennian, Qi, Jie, Liu, Jinxiang, Zhang, Quanqi
Format: Article
Language:English
Subjects:
Chromileptes altivelis
Expression profiles
Gene structure
Toll-like receptors
Vibrio harveyi
Online Access:Get full text
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Summary:Toll-like receptors (TLRs) represent a prominent category of pattern recognition receptors that have been extensively investigated for their pivotal role in combating pathogen incursions. Despite this, there has been a notable absence of comprehensive identification and exploration of the immune response associated with the TLR family genes in C. altivelis. This study successfully identified and named fourteen genes as Catlr1-1, Catlr1-2, Catlr2-1, Catlr2-2, Catlr3, Catlr5, Catlr7, Catlr8, Catlr9, Catlr13-1, Catlr13-2, Catlr18, Catlr21, and Catlr22. A series of bioinformatic analysis were performed, encompassing analysis of protein properties, examination of gene structures, evolutionary assessments, and prediction of protein tertiary structures. The expression patterns of Catlr genes were analyzed in five immune tissues: liver, spleen, kidney, gill, and intestine, in both healthy and bacterial stimulated-fish. The results showed that different tissue and different genes showed differed expression patterns after V. harveyi infection, indicating the involvement of all Catlr members in mounting immune responses following infection in various tissues. Additionally, histological evaluations of immune tissues unveiled varying levels of damage. In conclusion, this investigation into the TLR gene family offers novel information that contribute to a more profound comprehension of the immune response mechanisms in C. altivelis. [Display omitted] •Fourteen TLRs were identified, which showed diverse immune response patterns to Vibrio harveyi intra-peritoneally injected.•The TLR gene family experienced strong purifying selection pressure, resulting in limited functional divergence.•The TLR sequences in C. altivelis have demonstrated a high level of conservation throughout evolution.
ISSN:1050-4648
1095-9947
1095-9947
DOI:10.1016/j.fsi.2024.109720