Biosynthesis of gold nanoparticles by fungi and its potential in SERS

Surface enhanced Raman spectroscopy (SERS) by using gold nanoparticles (AuNPs) has gained relevance for the identification of biomolecules and some cancer cells. Searching for greener NPs synthesis alternatives, we evaluated the SERS properties of AuNPs produced by using different filamentous fungi....

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Bibliographic Details
Published in:Bioprocess and biosystems engineering 2024-09, Vol.47 (9), p.1585-1593
Main Authors: Olvera-Aripez, Jacqueline, Camacho-López, Santiago, Flores-Castañeda, Mariela, Belman-Rodríguez, Carlos, Vilchis-Nestor, Alfredo R., Castro-Longoria, Ernestina
Format: Article
Language:English
Subjects:
Alternaria
Biomolecules
Biosensors
Biosynthesis
Biotechnology
Chemical synthesis
Chemistry
Chemistry and Materials Science
Crystal structure
Environmental Engineering/Biotechnology
Evaluation
Food Science
Fungi
Gold
Industrial and Production Engineering
Industrial Chemistry/Chemical Engineering
Methylene blue
Nanoparticles
Raman spectroscopy
Research Paper
Spectroscopy
Spectrum analysis
Surface plasmon resonance
Transmission electron microscopy
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Summary:Surface enhanced Raman spectroscopy (SERS) by using gold nanoparticles (AuNPs) has gained relevance for the identification of biomolecules and some cancer cells. Searching for greener NPs synthesis alternatives, we evaluated the SERS properties of AuNPs produced by using different filamentous fungi. The AuNPs were synthesized utilizing the supernatant of Botrytis cinerea , Trichoderma atroviride , Trichoderma asperellum , Alternaria sp. and Ganoderma sessile . The AuNPs were characterized by ultraviolet–visible spectroscopy (UV–Vis) to identify its characteristic surface plasmon resonance, which was located at 545 nm ( B. cinerea ), 550 nm ( T. atroviride ), 540 nm ( T. asperellum ), 530 nm ( Alternaria sp.), and 525 nm ( G. sessile ). Morphology, size and crystal structure were characterized through transmission electron microscopy (TEM); colloidal stability was assessed by Z-potential measurements. We found that, under specific incubation conditions, it was possible to obtain AuNPs with spherical and quasi-spherical shapes, which mean size range depends on the fungal species supernatant with 92.9 nm ( B. cinerea ), 24.7 nm ( T. atroviride ), 16.4 nm ( T. asperellum ), 9.5 nm ( Alternaria sp.), and 13.6 nm ( G. sessile ). This, as it can be expected, has an effect on Raman amplification. A micro-Raman spectroscopy system operated at a wavelength of 532 nm was used for the evaluation of the SERS features of the AuNPs. We chose methylene blue as our target molecule since it has been widely used for such a purpose in the literature. Our results show that AuNPs synthesized with the supernatant of T. atroviride, T. asperellum and Alternaria sp. produce the stronger SERS effect, with enhancement factor (EF) of 20.9, 28.8 and 35.46, respectively. These results are promising and could serve as the base line for the development of biosensors through a facile, simple, and low-cost green alternative.
ISSN:1615-7591
1615-7605
1615-7605
DOI:10.1007/s00449-024-03053-w