Genome-wide association study and linkage mapping reveal TaqW-6B associated with water-extractable arabinoxylan content in wheat grain

Key message A novel QTL, TaqW-6B of water-extractable arabinoxylan content in the wheat grain on chromosome 6BL was identified and fine mapped in a narrow region 3.8 Mb. Water-extractable arabinoxylan (WE-AX), an important component of hemicellulose, is associated with various abundant health benefi...

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Bibliographic Details
Published in:Theoretical and applied genetics 2024-07, Vol.137 (7), p.166, Article 166
Main Authors: Li, Qiong, Zeng, Zhankui, Zhao, Yue, Li, Jiachuang, Chen, Feng, Wang, Chunping
Format: Article
Language:English
Subjects:
Agriculture
Biochemistry
Biomedical and Life Sciences
Biotechnology
Chromosomes
Gene mapping
Genome-wide association studies
Hemicellulose
Inbreeding
Life Sciences
Original Article
Phenotypic variations
Plant Biochemistry
Plant Breeding/Biotechnology
Plant Genetics and Genomics
Population genetics
Population studies
Quantitative trait loci
Single-nucleotide polymorphism
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Summary:Key message A novel QTL, TaqW-6B of water-extractable arabinoxylan content in the wheat grain on chromosome 6BL was identified and fine mapped in a narrow region 3.8 Mb. Water-extractable arabinoxylan (WE-AX), an important component of hemicellulose, is associated with various abundant health benefits. In this study, QTLs for WE-AX content were detected in two populations: (1) a recombinant inbred line (RIL) population with 164 lines derived from a cross between Avocet and Chilero (AC population) genotyped with diversity array technology (DArT), and (2) a natural population of 243 varieties (CH population) genotyped with the Axiom wheat 660 K single-nucleotide polymorphism (SNP) array. A stable QTL Qwe-ax.haust-6B , explaining 8.51–15.59% of the phenotypic variance, was mapped in the physical interval 459.38–572.09 Mb on the long arm of chromosome 6B in the AC population, tightly linked with DArT markers 3,944,740 and 4,991,038 under three experimental conditions. The Qwe-ax.haust-6B was further narrowed down to be delimited in the physical interval 516.47–571.58 Mb on chromosome 6BL, explaining 5.86–16.27% of the phenotypic variance in the CH population. Furthermore, we developed high-throughput kompetitive allele-specific PCR (KASP) markers to reconstruct the genetic linkage map in the AC population, and Qwe-ax.haust-6B was fine mapped into a narrow region named TaqW-6B , which was compressed between KASP-6B-3 and KASP-6B-6 at a physical distance of 3.8 Mb. In the meanwhile, the markers were also validated in a natural population of 160 wheat lines (NP population). Consequently, this study is of great importance to provide the theoretical basis for cloning the key gene and developing functional markers for molecular breeding.
ISSN:0040-5752
1432-2242
1432-2242
DOI:10.1007/s00122-024-04662-5