Upcycling CO2 into succinic acid via electrochemical and engineered Escherichia coli

[Display omitted] •Preparing Cu-OF catalyst to convert CO2 to ethanol (FE = 84.7 %, RP = 95 wt%).•Engineering E. coli metabolizes ethanol to efficiently produce succinic acid.•Succinic acid yield reached 0.41 mol/mol, which is 82 % of the theoretical yield. Converting CO2 into value-added chemicals...

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Bibliographic Details
Published in:Bioresource technology 2024-08, Vol.406, p.130956, Article 130956
Main Authors: Gong, Zhijin, Zhang, Wei, Chen, Jiayao, Li, Jingchuan, Tan, Tianwei
Format: Article
Language:English
Subjects:
Computational design
Cu-OF catalyst
Electrocatalytic CO2RR to ethanol
Metabolic engineering
Succinic acid production
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Summary:[Display omitted] •Preparing Cu-OF catalyst to convert CO2 to ethanol (FE = 84.7 %, RP = 95 wt%).•Engineering E. coli metabolizes ethanol to efficiently produce succinic acid.•Succinic acid yield reached 0.41 mol/mol, which is 82 % of the theoretical yield. Converting CO2 into value-added chemicals still remains a grand challenge. Succinic acid has long been considered as one of the top building block chemicals. This study reported efficiently upcycling CO2 into succinic acid by combining between electrochemical and engineered Escherichia coli. In this process, the Cu-organic framework catalyst was synthesized for electrocatalytic CO2-to-ethanol conversion with high Faradaic efficiency (FE, 84.7 %) and relative purity (RP, 95 wt%). Subsequently, an engineered E. coli with efficiently assimilating CO2-derived ethanol to produce succinic acid was constructed by combining computational design and metabolic engineering, and the succinic acid titer reached 53.8 mM with the yield of 0.41 mol/mol, which is 82 % of the theoretical yield. This study effort to link the two processes of efficient ethanol synthesis by electrocatalytic CO2 and succinic acid production from CO2-derived ethanol, paving a way for the production of succinic acid and other value-added chemicals by converting CO2 into ethanol.
ISSN:0960-8524
1873-2976
1873-2976
DOI:10.1016/j.biortech.2024.130956