Cationic lipid nanoparticle-mediated delivery of a Cas9/crRNA ribonucleoprotein complex for transgene-free editing of the citrus plant genome

Key message A lipofectamine-mediated transfection protocol for DNA-free genome editing of citrus protoplast cells using a Cas9/gRNA ribonucleoprotein (RNP) complex resulted in the production of transgene free genome edited citrus.

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Bibliographic Details
Published in:Plant cell reports 2024-07, Vol.43 (7), p.171, Article 171
Main Authors: Mahmoud, Lamiaa M., Dutt, Manjul
Format: Article
Language:English
Subjects:
Albinism
Biomedical and Life Sciences
Biotechnology
Cell Biology
Citrus fruits
Conflicts of interest
CRISPR
Editing
Embryos
Flowers & plants
Focus Article
Genome editing
Genomes
Genotype & phenotype
gRNA
Life Sciences
Lipids
Mutation
Nanoparticles
Plant Biochemistry
Plant Sciences
Polyethylene glycol
Regulatory approval
Transfection
Transgenes
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Description
Summary:Key message A lipofectamine-mediated transfection protocol for DNA-free genome editing of citrus protoplast cells using a Cas9/gRNA ribonucleoprotein (RNP) complex resulted in the production of transgene free genome edited citrus.
ISSN:0721-7714
1432-203X
1432-203X
DOI:10.1007/s00299-024-03254-3