Optimizing extraction methods by a comprehensive experimental approach and characterizing polyphenol compositions of Ecklonia radiata

Brown seaweed Ecklonia radiata harbors valuable polyphenols, notably phlorotannins, prized for their health benefits. This study optimized phlorotannin extraction via conventional solvent extraction and ultrasound-assisted extraction methods, utilizing variable concentrations of ethanol. Employing f...

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Bibliographic Details
Published in:Food chemistry 2024-10, Vol.455, p.139926, Article 139926
Main Authors: Duan, Xinyu, Subbiah, Vigasini, Agar, Osman Tuncay, Barrow, Colin J., Ashokkumar, Muthupandian, Dunshea, Frank R., Suleria, Hafiz A.R.
Format: Article
Language:English
Subjects:
Antioxidant
Ecklonia radiata
Optimization
Phlorotannins
Ultrasound-assisted extraction
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Summary:Brown seaweed Ecklonia radiata harbors valuable polyphenols, notably phlorotannins, prized for their health benefits. This study optimized phlorotannin extraction via conventional solvent extraction and ultrasound-assisted extraction methods, utilizing variable concentrations of ethanol. Employing fractional factorial designs, key variables were identified. Steepest ascent/descent method and central composite rotatable designs refined optimal conditions, enhancing phlorotannin and polyphenol yields, and antioxidant capacities. Under optimized conditions, phlorotannin contents reached 2.366 ± 0.01 and 2.596 ± 0.04 PGE mg/g, total polyphenol contents peaked at 10.223 ± 0.03 and 10.836 ± 0.02 GAE mg/g. Robust antioxidant activity was observed: DPPH and OH radical scavenging capacities measured 27.891 ± 0.06 and 17.441 ± 0.08 TE mg/g, and 37.498 ± 1.12 and 49.391 ± 0.82 TE mg/g, respectively. Reducing power capacities surged to 9.016 ± 0.02 and 28.110 ± 0.10 TE mg/g. Liquid chromatography-mass spectrometry (LC-MS) and high-performance liquid chromatography (HPLC) analyses revealed enriched antioxidant compounds. Variations in polyphenol profiles were noted, potentially influencing antioxidant capacity nuances. This study illuminated the potential of E. radiata potential as a polyphenol source and offers optimized extraction methods poised to benefit various industries. •Phenolic compounds with antioxidant effects were extracted from Ecklonia radiata.•Extraction parameters were meticulously optimized through experimental approaches.•Phenolic profiles and contents were compared between two extraction techniques.•Ultrasonic-assisted extraction showed superiority over traditional solvent method.
ISSN:0308-8146
1873-7072
1873-7072
DOI:10.1016/j.foodchem.2024.139926