Modification of the Hi-C Technology for Molecular Genetic Analysis of Formalin-Fixed Paraffin-Embedded Sections of Tumor Tissues

Molecular genetic analysis of tumor tissues is the most important step towards understanding the mechanisms of cancer development; it is also necessary for the choice of targeted therapy. The Hi-C (high-throughput chromatin conformation capture) technology can be used to detect various types of geno...

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Bibliographic Details
Published in:Biochemistry (Moscow) 2024-04, Vol.89 (4), p.637-652
Main Authors: Gridina, Maria M., Stepanchuk, Yana K., Nurridinov, Miroslav A., Lagunov, Timofey A., Torgunakov, Nikita Yu, Shadsky, Artem A., Ryabova, Anastasia I., Vasiliev, Nikolay V., Vtorushin, Sergey V., Gerashchenko, Tatyana S., Denisov, Evgeny V., Travin, Mikhail A., Korolev, Maxim A., Fishman, Veniamin S.
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Cancer
Chromatin
Chromosome rearrangements
Chromosome translocations
Chromosomes
Conformation
Formaldehyde
Genetic analysis
Genetic diversity
Genetic screening
Genetic testing
Inversions
Life Sciences
Medical prognosis
Medical treatment
Microbiology
Neoplasms
Paraffin
Paraffins
Precision medicine
Targeted cancer therapy
Tissue
Tumors
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Summary:Molecular genetic analysis of tumor tissues is the most important step towards understanding the mechanisms of cancer development; it is also necessary for the choice of targeted therapy. The Hi-C (high-throughput chromatin conformation capture) technology can be used to detect various types of genomic variants, including balanced chromosomal rearrangements, such as inversions and translocations. We propose a modification of the Hi-C method for the analysis of chromatin contacts in formalin-fixed paraffin-embedded (FFPE) sections of tumor tissues. The developed protocol allows to generate high-quality Hi-C data and detect all types of chromosomal rearrangements. We have analyzed various databases to compile a comprehensive list of translocations that hold clinical importance for the targeted therapy selection. The practical value of molecular genetic testing is its ability to influence the treatment strategies and to provide prognostic insights. Detecting specific chromosomal rearrangements can guide the choice of the targeted therapies, which is a critical aspect of personalized medicine in oncology.
ISSN:0006-2979
1608-3040
DOI:10.1134/S0006297924040047