A fluorescence probe with targeted mitochondria for detecting hydrogen peroxide in vitro and in diabetic mice

We designed and prepared probe W-1 for the detection of H O . W-1 showed excellent selectivity for H O and was accompanied by colorimetric signal changes. The excellent linear relationship between fluorescence intensity and H O concentration (0-100 μM) provided favorable conditions for its quantitat...

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Bibliographic Details
Published in:Analytical methods 2024-06, Vol.16 (24), p.3831-3838
Main Authors: Wang, Yi-Ru, Qiao, Fu-Qiang, Tan, Yu-Wei, Hu, Jia-Ling, Zhang, Ai-Hong, Liang, Ting, Liu, Xu-Ying, Song, Hong-Ru, Kang, Yan-Fei
Format: Article
Language:English
Subjects:
Animals
Colorimetry
Colorimetry - methods
Diabetes mellitus
Diabetes Mellitus, Experimental
Fluorescence
Fluorescent Dyes - chemistry
Fluorescent indicators
Humans
Hydrogen peroxide
Hydrogen Peroxide - metabolism
Mice
Mitochondria
Mitochondria - metabolism
Optical Imaging - methods
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Summary:We designed and prepared probe W-1 for the detection of H O . W-1 showed excellent selectivity for H O and was accompanied by colorimetric signal changes. The excellent linear relationship between fluorescence intensity and H O concentration (0-100 μM) provided favorable conditions for its quantitative detection. In addition, the combination of portable test strips with a smartphone platform provided great convenience for on-site visual detection of H O . Moreover, W-1 possessed targeting mitochondria property and could be applied to image the exogenous and endogenous H O in cells to distinguish normal cells and cancer cells. Lastly, W-1 was used for monitoring the H O fluctuation of the diabetic process in mice, and the results showed an increase in H O levels in diabetes. Therefore, the probe provided a tool for understanding the pathological and physiological mechanisms of diabetes by imaging H O .
ISSN:1759-9660
1759-9679
1759-9679
DOI:10.1039/d4ay00653d