Investigation of the biocontrol mechanism of a novel Pseudomonas species against phytopathogenic Fusarium graminearum revealed by multi-omics integration analysis

Phytopathogenic poses significant threats to crop health and soil quality. Although our laboratory-cultivated sp. P13 exhibited potential biocontrol capacities, its effectiveness against and underlying antifungal mechanisms are still unclear. In light of this, our study investigated a significant in...

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Published in:Applied and environmental microbiology 2024-05, Vol.90 (6), p.e0045524
Main Authors: Dai, Jiawei, Xu, Zhaofeng, Yang, Ning, Tuerxunjiang, Hamiguli, Shan, Xin, Diao, Yuting, Zhao, Jiahui, Ma, Meiqi, Li, Xiang, Xiao, Ming, Pei, Junmin
Format: Article
Language:English
Subjects:
Applied and Industrial Microbiology
Biological control
Biosynthesis
Environmental Microbiology
Fungicides
Fusarium graminearum
Growth stage
Hydrogen cyanide
Metabolic pathways
Metabolomics
Pseudomonas
Pyoverdines
Siderophores
Soil environment
Soil quality
Transcriptomics
Tricarboxylic acid cycle
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Summary:Phytopathogenic poses significant threats to crop health and soil quality. Although our laboratory-cultivated sp. P13 exhibited potential biocontrol capacities, its effectiveness against and underlying antifungal mechanisms are still unclear. In light of this, our study investigated a significant inhibitory effect of P13 on T1, both and in a soil environment. Conducting genomic, metabolomic, and transcriptomic analyses of P13, we sought to identify evidence supporting its antagonistic effects on T1. The results revealed the potential of P13, a novel species, to produce active antifungal components, including phenazine-1-carboxylate (PCA), hydrogen cyanide (HCN), and siderophores [pyoverdine (Pvd) and histicorrugatin (Hcs)], as well as the dynamic adaptive changes in the metabolic pathways of P13 related to these active ingredients. During the logarithmic growth stage, T1 exposed P13 strategically upregulated PCA and HCN biosynthesis, along with transient inhibition of the tricarboxylic acid (TCA) cycle. However, with growth stabilization, upregulation of PCA and HCN synthesis ceased, whereas the TCA cycle was enhanced, increasing siderophores secretion (Pvd and Hcs), suggesting that this mechanism might have caused continuous inhibition of T1. These findings improved our comprehension of the biocontrol mechanisms of P13 and provided the foundation for potential application of strains in the biocontrol of phytopathogenic . spp. produces various antifungal substances, making it an effective natural biocontrol agent against pathogenic fungi. However, the inhibitory effects and the associated antagonistic mechanisms of spp. against spp. are unclear. Multi-omics integration analyses of the antifungal effects of novel species, P13, against T1 revealed the ability of P13 to produce antifungal components (PCA, HCN, Pvd, and Hcs), strategically upregulate PCA and HCN biosynthesis during logarithmic growth phase, and enhance the TCA cycle during stationary growth phase. These findings improved our understanding of the biocontrol mechanisms of P13 and its potential application against pathogenic fungi.
ISSN:0099-2240
1098-5336
1098-5336
DOI:10.1128/aem.00455-24