The use of bioluminescent enzyme bioassay for the analysis of human saliva: Advantages and disadvantages

The purpose of the work was to find optimal conditions for bioluminescent enzymatic analysis of saliva (based on the use of NADH:FMN oxidoreductase + luciferase) and then to determine the biological effect of using bioluminescence assay of saliva to study the physiological state of the body under no...

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Bibliographic Details
Published in:Luminescence (Chichester, England) England), 2024-05, Vol.39 (5), p.e4776-n/a
Main Authors: Kolenchukova, Oksana A., Dedora, Anastasia O., Stepanova, Lyudmila V., Kravchuk, Vlada U., Kratasyuk, Valentina A.
Format: Article
Language:English
Subjects:
Bio-assays
Bioassays
Biological Assay
Biological effects
Bioluminescence
biomarkers
Cortisol
Enzyme immunoassay
Enzymes
Hormones
Humans
Hydrocortisone - analysis
Hydrocortisone - metabolism
Immunoassay
Immunoassays
Immunoglobulin A
Immunoglobulin A, Secretory - analysis
Immunoglobulin A, Secretory - metabolism
Luciferases - chemistry
Luciferases - metabolism
Luminescence
Luminescent Measurements - methods
NADH:FMN oxidoreductase + reductase
Nicotinamide adenine dinucleotide
Oxidoreductase
Oxidoreductases
Pharyngitis
Physiological effects
Saliva
Saliva - chemistry
Saliva - enzymology
Spectrophotometry
Training
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Summary:The purpose of the work was to find optimal conditions for bioluminescent enzymatic analysis of saliva (based on the use of NADH:FMN oxidoreductase + luciferase) and then to determine the biological effect of using bioluminescence assay of saliva to study the physiological state of the body under normal and pathological conditions. The saliva of snowboarders and students were studied in the “rest–training” model. The saliva of patients diagnosed with acute pharyngitis was examined in the “sick–healthy” model. Bioluminescence assay was performed with a lyophilized and immobilized bi‐enzyme system using cuvette, plate, and portable luminometers. The concentrations of secretory immunoglobulin A (sIgA) and cortisol were determined by enzyme immunoassay, and the total protein content was measured by spectrophotometric method. The activity of the bioluminescent system enzymes increased as the amount and volume of saliva in the sample was decreased. The cuvette and plate luminometers were sensitive to changes in the luminescence intensity in saliva assay. Luminescence intensity correlated with the concentrations of sIgA and cortisol. The integrated bioluminescent index for saliva was reduced in the “rest–training” model and increased in the “sick–healthy” model. Thus, the non‐invasive bioluminescent saliva analysis may be a promising tool for assessing the health of the population. The work is devoted to the study of the mechanisms of bioluminescent testing of saliva, depending on the type of luminometer, the amount of saliva, and the effect of its metabolites on bioluminescent glow, as well as the possibility of testing saliva by bioluminescent method during exercise and in the presence of pathology.
ISSN:1522-7235
1522-7243
DOI:10.1002/bio.4776