A Highly Sensitive and Selective Fluorescent Sensor for Folic Acid Detection Based on D‐penicillamine Stabilized Ag/Cu Alloy Nanoclusters

In this work, a highly sensitive and selective method for detecting folic acid (FA) was developed using D‐penicillamine (DPA) stabilized Ag/Cu alloy nanoclusters (DPA@Ag/Cu NCs). The yellow emission of DPA@Ag/Cu NCs was found to be quenched upon the addition of FA to the system. The fluorescence int...

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Bibliographic Details
Published in:Chembiochem : a European journal of chemical biology 2024-07, Vol.25 (14), p.e202400254-n/a
Main Authors: Mei Zhang, Sai, Xue Dong, Jiang, Li Wu, Xiao, Sen Zhao, Yong, Lei Li, Yan, Lin Wang, Shou, Yang, Yang, An, Miao, Su, Ming, Ya Shi, Rong, Feng Gao, Zhong
Format: Article
Language:English
Subjects:
Absorption spectra
Alloy nanoclusters
Amino acids
Biological effects
Biological properties
Biological samples
Copper
Copper base alloys
Electron transfer
Fluorescence
Fluorescence lifetime sensing devices
Fluorescent sensor
Folic acid
Functional nanosensor
High resolution electron microscopy
Nanoalloys
Nanoclusters
Quenching
Silver
Tablets
Transmission electron microscopy
Ultraviolet spectra
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Summary:In this work, a highly sensitive and selective method for detecting folic acid (FA) was developed using D‐penicillamine (DPA) stabilized Ag/Cu alloy nanoclusters (DPA@Ag/Cu NCs). The yellow emission of DPA@Ag/Cu NCs was found to be quenched upon the addition of FA to the system. The fluorescence intensity quenching value demonstrated a linear relationship with FA concentrations ranging from 0.01 to 1200 μM, with a limit of detection (LOD) of 5.3 nM. Furthermore, the detection mechanism was investigated through various characterization analyses, including high resolution transmission electron microscopy, fluorescence spectra, ultraviolet‐visible absorption spectra, and fluorescence lifetime. The results indicated that the fluorescence quenching induced by FA was a result of electron transfer from FA to the ligands of DPA@Ag/Cu NCs. The selectivity of the FA sensor was also evaluated, showing that common amino acids and inorganic ions had minimal impact on the detection of FA. Moreover, the standard addition method was successfully applied to detect FA in human serum, chewable tablets and FA tablets with promising results. The use of DPA@Ag/Cu NCs demonstrates significant potential for detecting FA in complex biological samples. In this work, a sensor was developed for detecting folic acid (FA) using D‐penicillamine stabilized Ag/Cu alloy nanoclusters (DPA@Ag/Cu NCs). Experimental results demonstrated a linear relationship between FA concentrations and fluorescence quenching of DPA@Ag/Cu NCs, with a low detection limit of 5.3 nM, based on the fluorescence quenching mechanism caused by electron transfer. Moreover, this approach was successfully applied to FA in complex biological samples.
ISSN:1439-4227
1439-7633
1439-7633
DOI:10.1002/cbic.202400254