Accelerated diagnosis: a crosslinking catalytic hairpin assembly system for rapid and sensitive SARS-CoV-2 RNA detection

The COVID-19 pandemic has underscored the urgent need for rapid and reliable strategies for early detection of SARS-CoV-2. In this study, we propose a DNA nanosphere-based crosslinking catalytic hairpin assembly (CCHA) system for the rapid and sensitive SARS-CoV-2 RNA detection. The CCHA system empl...

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Bibliographic Details
Published in:Mikrochimica acta (1966) 2024-06, Vol.191 (6), p.333-333, Article 333
Main Authors: Mo, Liuting, Yuan, Rongzheng, Hong, Yan, Yang, Chan, Lin, Weiying
Format: Article
Language:English
Subjects:
Analytical Chemistry
Animals
Assembly
Cattle
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
COVID-19
COVID-19 - diagnosis
COVID-19 - virology
COVID-19 Nucleic Acid Testing - methods
Cross-Linking Reagents - chemistry
Crosslinking
Deoxyribonucleic acid
Diagnosis
DNA
DNA - chemistry
Humans
Inverted Repeat Sequences
Limit of Detection
Microengineering
Nanochemistry
Nanospheres
Nanospheres - chemistry
Nanotechnology
Original Paper
Reagents
Ribonucleic acid
RNA
RNA, Viral - analysis
RNA, Viral - genetics
Saliva - virology
SARS-CoV-2 - genetics
SARS-CoV-2 - isolation & purification
Severe acute respiratory syndrome coronavirus 2
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Summary:The COVID-19 pandemic has underscored the urgent need for rapid and reliable strategies for early detection of SARS-CoV-2. In this study, we propose a DNA nanosphere-based crosslinking catalytic hairpin assembly (CCHA) system for the rapid and sensitive SARS-CoV-2 RNA detection. The CCHA system employs two DNA nanospheres functionalized with catalytic hairpin assembly (CHA) hairpins. The presence of target SARS-CoV-2 RNA initiated the crosslinking of DNA nanospheres via CHA process, leading to the amplification of fluorescence signals. As a result, the speed of SARS-CoV-2 diagnosis was enhanced by significantly increasing the local concentration of the reagents in a crosslinked DNA product, leading to a detection limit of 363 fM within 5 min. The robustness of this system has been validated in complex environments, such as fetal bovine serum and saliva. Hence, the proposed CCHA system offers an efficient and simple approach for rapid detection of SARS-CoV-2 RNA, holding substantial promise for enhancing COVID-19 diagnosis. Graphical Abstract
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-024-06396-z