Screening for brown-spot disease and drought stress response and identification of dual-stress responsive genes in rice cultivars of Northeast India

Rice cultivation in Northeast India (NEI) primarily relies on rainfed conditions, making it susceptible to severe drought spells that promote the onset of brown spot disease (BSD) caused by Bipolaris oryzae . This study investigates the response of prevalent rice cultivars of NEI to the combined str...

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Bibliographic Details
Published in:Physiology and molecular biology of plants 2024-04, Vol.30 (4), p.647-663
Main Authors: Das, Debajit, Chowdhury, Naimisha, Sharma, Monica, Suma, Remya, Saikia, Banashree, Velmurugan, Natarajan, Chikkaputtaiah, Channakeshavaiah
Format: Article
Language:English
Subjects:
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Brown spot
Catalase
Cell Biology
Combined stress
Cultivars
Cultivation
Defense mechanisms
Drought
Drought resistance
Electrolyte leakage
Enzymatic activity
Enzyme activity
Enzymes
Genes
Grain cultivation
Guaiacol
Life Sciences
Lipid peroxidation
Lipids
Mitigation
Nucleotide sequence
PCR
Peroxidase
Peroxidation
Plant Physiology
Plant Sciences
Proline
Reactive oxygen species
Research Article
Rice
Stress response
Transcriptomics
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Summary:Rice cultivation in Northeast India (NEI) primarily relies on rainfed conditions, making it susceptible to severe drought spells that promote the onset of brown spot disease (BSD) caused by Bipolaris oryzae . This study investigates the response of prevalent rice cultivars of NEI to the combined stress of drought and B. oryzae infection. Morphological, physiological, biochemical, and molecular changes were recorded post-stress imposition. Qualitative assessment of reactive oxygen species through DAB (3,3-diaminobenzidine) assay confirmed the elicitation of plant defense responses. Based on drought scoring system and biochemical analyses, the cultivars were categorized into susceptible (Shasharang and Bahadur), moderately susceptible (Gitesh and Ranjit), and moderately tolerant (Kapilee and Mahsuri) groups. Antioxidant enzyme accumulation (catalase, guaiacol peroxidase) and osmolyte (proline) levels increased in all stressed plants, with drought-tolerant cultivars exhibiting higher enzyme activities, indicating stress mitigation efforts. Nevertheless, electrolyte leakage and lipid peroxidation rates increased in all stressed conditions, though variations were observed among stress types. Based on findings from a previous transcriptomic study, a total of nine genes were chosen for quantitative real-time PCR analysis. Among these, OsEBP89 appeared as a potential negative regulatory gene, demonstrating substantial upregulation in the susceptible cultivars at both 48 and 72 h post-treatment (hpt). This finding suggests that OsEBP89 may play a role in conferring drought-induced susceptibility to BSD in the rice cultivars being investigated.
ISSN:0971-5894
0974-0430
DOI:10.1007/s12298-024-01447-4