Massive field-of-view sub-cellular traction force videography enabled by Single-Pixel Optical Tracers (SPOT)

We report a massive field-of-view and high-speed videography platform for measuring the sub-cellular traction forces of more than 10,000 biological cells over 13 mm2 at 83 frames per second. Our Single-Pixel Optical Tracers (SPOT) tool uses 2-dimensional diffraction gratings embedded into a soft sub...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2024-08, Vol.258, p.116318-116318, Article 116318
Main Authors: Marvin Tan, Xing Haw, Wang, Yijie, Zhu, Xiongfeng, Mendes, Felipe Nanni, Chung, Pei-Shan, Chow, Yu Ting, Man, Tianxing, Lan, Hsin, Lin, Yen-Ju, Zhang, Xiang, Zhang, Xiaohe, Nguyen, Thang, Ardehali, Reza, Teitell, Michael A., Deb, Arjun, Chiou, Pei-Yu
Format: Article
Language:English
Subjects:
Animals
Bio-MEMS
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Cell traction force
Cells, Cultured
Diffraction grating
Equipment Design
Myocytes, Cardiac - cytology
Rats
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Summary:We report a massive field-of-view and high-speed videography platform for measuring the sub-cellular traction forces of more than 10,000 biological cells over 13 mm2 at 83 frames per second. Our Single-Pixel Optical Tracers (SPOT) tool uses 2-dimensional diffraction gratings embedded into a soft substrate to convert cells' mechanical traction force into optical colors detectable by a video camera. The platform measures the sub-cellular traction forces of diverse cell types, including tightly connected tissue sheets and near isolated cells. We used this platform to explore the mechanical wave propagation in a tightly connected sheet of Neonatal Rat Ventricular Myocytes (NRVMs) and discovered that the activation time of some tissue regions are heterogeneous from the overall spiral wave behavior of the cardiac wave.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2024.116318