Cutaneous glands of the striped toad, Rhinella crucifer (Wied-Neuwied, 1821) (Amphibia: Bufonidae): Histological study and bioactivities of glandular secretions

This study investigated the morphology of Rhinella crucifer cutaneous glands, as well as the protein/peptide profiles and bioactivities of body gland secretions (BGS) and parotoid macrogland secretions (PS). The parotoid as well as dorsal and ventral skin fragments of male and female individuals wer...

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Published in:Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2024-08, Vol.273, p.110983-110983, Article 110983
Main Authors: Barbosa, Géssica Gomes, Silva, Tulíbia Laurindo, de Oliveira, Ana Patrícia Silva, de Albuquerque Lima, Thâmarah, da Silva, Pollyanna Michelle, de Santana, Carlos José Correia, Vieira, Jeymesson Raphael Cardoso, de Sousa, Dyeime Ribeiro, Souza, Francisco Assis Leite, Pereira, Rafael, Zingali, Russolina Benedeta, Costa, Romero Marcos Pedrosa Brandão, Paiva, Patrícia Maria Guedes, Rodrigues, Gilberto Gonçalves, Castro, Mariana S., Napoleão, Thiago Henrique
Format: Article
Language:English
Subjects:
Amphibian Proteins - metabolism
Amphibian Proteins - pharmacology
Amphibian skin glands
Animals
Anticoagulant
Bodily Secretions - chemistry
Bufonidae - metabolism
Exocrine Glands - metabolism
Female
Male
Morphology
Protease
Skin - chemistry
Skin - metabolism
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Summary:This study investigated the morphology of Rhinella crucifer cutaneous glands, as well as the protein/peptide profiles and bioactivities of body gland secretions (BGS) and parotoid macrogland secretions (PS). The parotoid as well as dorsal and ventral skin fragments of male and female individuals were processed for histological analysis. The protein and peptide profiles of male and female gland secretions were evaluated. Male secretions were also assessed for proteolytic, trypsin inhibiting, hemagglutinating, hemolytic, antimicrobial, and anticoagulant activities. The R. crucifer skin structure presented protuberances that are clearly visible and formed by the integument, which has cutaneous glands throughout the body. An average of 438 and 333 glands were identified in males in females, respectively. No significant differences were observed in the distribution of glands across the body as well as for area and perimeter of glands. Differences were observed in protein composition between the PS and BGS from males and females, and secretions from animals collected from undisturbed and anthropogenically disturbed areas. Proteins with similarities to catalase and elongation factor 1-alpha were detected in the PS. Zymography revealed proteolytic activity in both male BGS and PS. Male BGS showed antibacterial activity against Enterococcus faecalis and Escherichia coli and anticoagulant activity, being able to prolong prothrombin time by 6.34-fold and activated partial thromboplastin time by 2.17-fold. Finally, male PS and BGS caused a maximum hemolysis degree of 1.4%. The data showed that the cutaneous secretions of R. crucifer are potentially promising for biotechnological prospecting. [Display omitted] •R. crucifer has an irregular skin formed by an integument that has cutaneous glands.•Differences were observed in protein composition in secretion of body glands and parotoid macroglands.•Secretions from males and females showed difference in composition.•Proteins with similarities to catalase and EF-1α were detected in the parotoid secretion.•Cutaneous gland secretions of R. crucifer are sources of proteases, anticoagulants, and antibacterial agents.
ISSN:1096-4959
1879-1107
DOI:10.1016/j.cbpb.2024.110983