Dual crosslinking silk fibroin/pectin-based bioink development and the application on neural stem/progenitor cells spheroid laden 3D bioprinting

The human nervous system is an incredibly intricate physiological network, and neural cells lack the ability to repair and regenerate after a brain injury. 3-dimensional (3D) bioprinting technology offers a promising strategy for constructing biomimetic organ constructs and in vitro brain/disease mo...

Full description

Saved in:
Bibliographic Details
Published in:International journal of biological macromolecules 2024-06, Vol.269 (Pt 2), p.131720-131720, Article 131720
Main Authors: Lee, Hao-Wei, Chen, Ko-Ting, Li, Yi-Chen Ethan, Yeh, Yu-Chun, Chiang, Chao-Ying, Lee, I-Chi
Format: Article
Language:English
Subjects:
3D bioprinting
Animals
Biocompatible Materials - chemistry
Biocompatible Materials - pharmacology
Bioink
Bioprinting - methods
Cell Differentiation - drug effects
Fibroins - chemistry
Humans
In vitro brain model
Ink
Neural Stem Cells - cytology
Neural Stem Cells - drug effects
Neural Stem Cells - metabolism
Neural stem/progenitor cells (NSPCs)
Pectin
Pectins - chemistry
Printing, Three-Dimensional
Silk fibroin/methacrylated silk fibroin (SilMA)
Spheroids, Cellular - cytology
Tissue Engineering - methods
Tissue Scaffolds - chemistry
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The human nervous system is an incredibly intricate physiological network, and neural cells lack the ability to repair and regenerate after a brain injury. 3-dimensional (3D) bioprinting technology offers a promising strategy for constructing biomimetic organ constructs and in vitro brain/disease models. The bioink serves as a pivotal component that emulates the microenvironment of biomimetic construct and exerts a profound influence on cellular behaviors. In this study, a series of mechanically adjustable and dual crosslinking bioinks were developed using photocrosslinkable methacrylated silk fibroin (SilMA) in combination with the ionic crosslinking material, pectin, or pectin methacryloyl (PecMA) with silk fibroin (SF) supplementation. SilMA/pectin exhibited superior properties, with SilMA providing biocompatibility and adjustable mechanical properties, while the addition of pectin enhanced printability. The porous structure supported neural cell growth, and 15 % SilMA/0.5 % pectin bioinks displayed excellent printability and shape fidelity. Neural stem/progenitor cells (NSPCs)-loaded bioinks were used to construct a 3D brain model, demonstrating sustained vitality and high neuronal differentiation without the need for growth factors. The SilMA/pectin bioinks demonstrated adjustable mechanical properties, favorable biocompatibility, and an environment highly conducive to neural induction, offering an alternative approach for neural tissue engineering applications or in vitro brain models. •A series of mechanically adjustable and dual crosslinking bioinks were developed and compared in this study.•The Sil-MA/Pectin bioinks demonstrated adjustable mechanical properties, favorable biocompatibility, and an environment highly conducive to neural induction on the NSPCs spheroid laden 3D bioprinting.•The Sil-MA/Pectin bioinks offering an alternative approach for neural tissue engineering applications or in vitro brain models.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2024.131720