Study on the interaction between erythrosine B and the cardiac drug amiodarone using fluorescence, scattering, and absorbance spectra and their analytical application

In an acidic buffered solution, erythrosine B can react with amiodarone to form an association complex, which not only generates great enhancement in resonance Rayleigh scattering (RRS) spectrum of erythrosine B at 346.5 nm but also results in quenching of fluorescence spectra of erythrosine B at λe...

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Bibliographic Details
Published in:Luminescence (Chichester, England) England), 2024-04, Vol.39 (4), p.e4748-n/a
Main Authors: AlSalem, Huda Salem, Alharbi, Sara Naif, Binkadem, Mona Saad, Mahmoud, Shimaa A., Abdel‐Lateef, Mohamed A.
Format: Article
Language:English
Subjects:
Absorbance
Amiodarone
Amiodarone - analysis
Amiodarone - chemistry
amiodarone hydrochloride
Anti-Arrhythmia Agents - analysis
Anti-Arrhythmia Agents - chemistry
Erythrosine - analysis
Erythrosine - chemistry
erythrosine B
Fluorescence
Molecular Structure
Rayleigh scattering
resonance Rayleigh scattering
Resonance scattering
Spectra
Spectrometry, Fluorescence
Spectrophotometry
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Summary:In an acidic buffered solution, erythrosine B can react with amiodarone to form an association complex, which not only generates great enhancement in resonance Rayleigh scattering (RRS) spectrum of erythrosine B at 346.5 nm but also results in quenching of fluorescence spectra of erythrosine B at λemission = 550.4 nm/λexcitation = 528.5 nm. In addition, the formed erythrosine B–amiodarone complex produces a new absorbance peak at 555 nm. The spectral characteristics of the RRS, absorbance, and fluorescence spectra, as well as the optimum analytical conditions, were studied and investigated. As a result, new spectroscopic methods were developed to determine amiodarone by utilizing erythrosine B as a probe. Moreover, the ICH guidelines were used to validate the developed RRS, photometric, and fluorimetric methods. The enhancements in the absorbance and the RRS intensity and the decrease in the fluorescence intensity of the used probe were proportional to the concentration of amiodarone in ranges of 2.5–20.0, 0.2–2.5, and 0.25–1.75 μg/mL, respectively. Furthermore, limit of detection values were 0.52 ng/mL for the spectrophotometric method, 0.051 μg/mL for the RRS method, and 0.075 μg/mL for the fluorimetric method. Moreover, with good recoveries, the developed spectroscopic procedures were applied to analyze amiodarone in its commercial tablets. Determination of amiodarone by spectrofluorimetric, scattering, and spectrophotometric methods based on its interaction with erythrosine B.
ISSN:1522-7235
1522-7243
DOI:10.1002/bio.4748