Characterization of a novel mouse platelet transfusion model

Background and Objectives Platelet transfusions are increasing with medical advances. Based on FDA criteria, platelet units are assessed by in vitro measures; however, it is not known how platelet processing and storage duration affect function in vivo. Our study's aim was to develop a novel pl...

Full description

Saved in:
Bibliographic Details
Published in:Vox sanguinis 2024-07, Vol.119 (7), p.702-711
Main Authors: Gordy, Dominique, Swayne, Theresa, Berry, Gregory J., Thomas, Tiffany A., Hudson, Krystalyn E., Stone, Elizabeth F.
Format: Article
Language:English
Subjects:
Adenosine diphosphate
Blood platelets
Cell activation
Criteria
In vivo methods and tests
mouse model
platelet
platelet activation
platelet aggregation
platelet storage
platelet transfusion
Platelets
Storage conditions
Thrombin
Transfusion
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background and Objectives Platelet transfusions are increasing with medical advances. Based on FDA criteria, platelet units are assessed by in vitro measures; however, it is not known how platelet processing and storage duration affect function in vivo. Our study's aim was to develop a novel platelet transfusion model stored in mouse plasma that meets FDA criteria adapted to mice, and transfused fresh and stored platelets are detectable in clots in vivo. Study Design and Methods Platelet units stored in mouse plasma were prepared using a modified platelet‐rich plasma (PRP) collection protocol. Characteristics of fresh and stored units, including pH, cell count, in vitro measures of activity, including activation and aggregation, and post‐transfusion recovery (PTR), were determined. Lastly, a tail transection assay was conducted using mice transfused with fresh or stored units, and transfused platelets were identified by confocal imaging. Results Platelet units had acceptable platelet and white cell counts and were negative for bacterial contamination. Fresh and 1‐day stored units had acceptable pH; the platelets were activatable by thrombin and adenosine diphosphate, agreeable with thrombin, had acceptable PTR, and were present in vivo in clots of recipients after tail transection. In contrast, 2‐day stored units had clinically unacceptable quality. Conclusion We developed mouse platelets for transfusion analogous to human platelet units using a modified PRP collection protocol with maximum storage of 1 day for an ‘old’ unit. This provides a powerful tool to test how process modifications and storage conditions affect transfused platelet function in vivo.
ISSN:0042-9007
1423-0410
1423-0410
DOI:10.1111/vox.13642