Study on the hemodynamic effects of different pulsatile working modes of a rotary blood pump using a microfluidic platform that realizes in vitro cell culture effectively

Rotary blood pumps (RBPs) operating at a constant speed generate non-physiologic blood pressure and flow rate, which can cause endothelial dysfunction, leading to adverse clinical events in peripheral blood vessels and other organs. Notably, pulsatile working modes of the RBP can increase vascular p...

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Bibliographic Details
Published in:Lab on a chip 2024-04, Vol.24 (9), p.2428-2439
Main Authors: Liang, Lixue, Wang, Xueying, Chen, Dong, Sethu, Palaniappan, Giridharan, Guruprasad A, Wang, Yanxia, Wang, Yu, Qin, Kai-Rong
Format: Article
Language:English
Subjects:
Blood pressure
Blood pumps
Blood vessels
Cell culture
Cell Culture Techniques - instrumentation
Cells, Cultured
Endothelial cells
Endothelial Cells - cytology
Endothelial Cells - metabolism
Equipment Design
Heart-Assist Devices
Hemodynamics
Human Umbilical Vein Endothelial Cells - metabolism
Humans
Lab-On-A-Chip Devices
Microfluidic Analytical Techniques - instrumentation
Nitric oxide
Nitric Oxide - metabolism
Pulsatile Flow
Reactive Oxygen Species - metabolism
Shear stress
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Summary:Rotary blood pumps (RBPs) operating at a constant speed generate non-physiologic blood pressure and flow rate, which can cause endothelial dysfunction, leading to adverse clinical events in peripheral blood vessels and other organs. Notably, pulsatile working modes of the RBP can increase vascular pulsatility to improve arterial endothelial function. However, the laws and related mechanisms of differentially regulating arterial endothelial function under different pulsatile working modes are still unclear. This knowledge gap hinders the optimal selection of the RBP working modes. To address these issues, this study developed a multi-element endothelial cell culture system (ECCS), which could realize cell culture effectively and accurately reproduce blood pressure, shear stress, and circumferential strain in the arterial endothelial microenvironment. Performance of this proposed ECCS was validated with numerical simulation and flow experiments. Subsequently, this study investigated the effects of four different pulsation frequency modes that change once every 1-4-fold cardiac cycles (80, 40, 80/3, and 20 cycles per min, respectively) of the RBP on the expression of nitric oxide (NO) and reactive oxygen species (ROS) in endothelial cells. Results indicated that the 2-fold and 3-fold cardiac cycles significantly increased the production of NO and prevented the excessive generation of ROS, potentially minimizing the occurrence of endothelial dysfunction and related adverse events during the RBP support, and were consistent with animal study findings. In general, this study may provide a scientific basis for the optimal selection of the RBP working modes and potential treatment options for heart failure.
ISSN:1473-0197
1473-0189
1473-0189
DOI:10.1039/d4lc00159a