Unravelling inulin molecules in food sources using a matrix-assisted laser desorption/ionization magnetic resonance mass spectrometry (MALDI-MRMS) pipeline

[Display omitted] •CHCA-BA is an optimal matrix for analyzing inulin extracts in plants by MALDI-MRMS.•Chicory inulin powder is an appropriate inulin standard for quantifying DP 9–22.•MALDI-MRMS can rapidly and reliably quantify each DP of inulin in plants.•The DP range length ranked as follows: ele...

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Published in:Food research international 2024-05, Vol.184, p.114276-114276, Article 114276
Main Authors: Chin, Sung-Tong, Boughton, Berin A., Gay, Melvin C.L., Russell, Alyce C., Wang, Yimin, Nambiar, Vimalnath, McHenry, Mark P., Holmes, Elaine, Nicholson, Jeremy K., Loo, Ruey Leng
Format: Article
Language:English
Subjects:
Antioxidants
Biological Products
Carbohydrate
Cynara scolymus
Fructans
Garlic
Helianthus
Inulin
Lasers
Magnetic Resonance Spectroscopy
Matrix-assisted laser desorption/ionization magnetic resonance mass spectrometry (MALDI-MRMS)
Prebiotics
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Summary:[Display omitted] •CHCA-BA is an optimal matrix for analyzing inulin extracts in plants by MALDI-MRMS.•Chicory inulin powder is an appropriate inulin standard for quantifying DP 9–22.•MALDI-MRMS can rapidly and reliably quantify each DP of inulin in plants.•The DP range length ranked as follows: elephant garlic > globe artichoke > Jerusalem artichoke > yacón.•The inulin content ratio, relative to globe artichoke, is 1:2:3 for yacón, elephant garlic and Jerusalem artichoke. Inulin, a polysaccharide characterized by a β-2,1 fructosyl-fructose structure terminating in a glucosyl moiety, is naturally present in plant roots and tubers. Current methods provide average degrees of polymerization (DP) but lack information on the distribution and absolute concentration of each DP. To address this limitation, a reproducible (CV 
ISSN:0963-9969
1873-7145
DOI:10.1016/j.foodres.2024.114276