Profile of miRNAs in small extracellular vesicles released from glioblastoma cells treated by boron neutron capture therapy

Purpose Boron neutron capture therapy (BNCT) is a tumor cell-selective particle-radiation therapy. In BNCT, administered p -boronophenylalanine (BPA) is selectively taken up by tumor cells, and the tumor is irradiated with thermal neutrons. High-LET α-particles and recoil 7 Li, which have a path len...

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Bibliographic Details
Published in:Journal of neuro-oncology 2024-05, Vol.168 (1), p.91-97
Main Authors: Kondo, Natsuko, Kinouchi, Tadatoshi, Natsumeda, Manabu, Matsuzaki, Juntaro, Hirata, Eishu, Sakurai, Yoshinori, Okada, Masayasu, Suzuki, Minoru
Format: Article
Language:English
Subjects:
Body fluids
Boron
Boron Neutron Capture Therapy - methods
Brain Neoplasms - genetics
Brain Neoplasms - metabolism
Brain Neoplasms - pathology
Brain Neoplasms - radiotherapy
Brain tumors
Carbon dioxide
Cell culture
Cell Line, Tumor
Column chromatography
Culture media
Extracellular vesicles
Extracellular Vesicles - metabolism
Extracellular Vesicles - radiation effects
Gene Expression Regulation, Neoplastic - radiation effects
Glioblastoma
Glioblastoma - genetics
Glioblastoma - metabolism
Glioblastoma - pathology
Glioblastoma - radiotherapy
Glioblastoma cells
Glioma
Humans
Medical prognosis
Medicine
Medicine & Public Health
MicroRNAs
MicroRNAs - genetics
MicroRNAs - metabolism
miRNA
Neurology
Neutrons
Oncology
Prognosis
Radiation
Radiation therapy
Survival analysis
Tumor cells
Citations: Items that this one cites
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Summary:Purpose Boron neutron capture therapy (BNCT) is a tumor cell-selective particle-radiation therapy. In BNCT, administered p -boronophenylalanine (BPA) is selectively taken up by tumor cells, and the tumor is irradiated with thermal neutrons. High-LET α-particles and recoil 7 Li, which have a path length of 5–9 μm, are generated by the capture reaction between 10 B and thermal neutrons and selectively kill tumor cells that have uptaken 10 B. Although BNCT has prolonged the survival time of malignant glioma patients, recurrences are still to be resolved. miRNAs, that are encapsulated in small extracellular vesicles (sEVs) in body fluids and exist stably may serve critical role in recurrence. In this study, we comprehensively investigated microRNAs (miRNAs) in sEVs released from post-BNCT glioblastoma cells. Method Glioblastoma U87 MG cells were treated with 25 ppm of BPA in the culture media and irradiated with thermal neutrons. After irradiation, they were plated into dishes and cultured for 3 days in the 5% CO 2 incubator. Then, sEVs released into the medium were collected by column chromatography, and miRNAs in sEVs were comprehensively investigated using microarrays. Result An increase in 20 individual miRNAs (ratio > 2) and a decrease in 2 individual miRNAs (ratio 
ISSN:0167-594X
1573-7373
1573-7373
DOI:10.1007/s11060-024-04649-8