A rapid and sensitive LC–MS/MS method for quantifying oxycodone, noroxycodone, oxymorphone and noroxymorphone in human plasma to support pharmacokinetic drug interaction studies of oxycodone

A sensitive and reliable LC–MS/MS method was developed and validated for the quantification of oxycodone and metabolites in human plasma. The method has a runtime of 6 min and a sensitivity of 0.1 μg/L for all analytes. Sample preparation consisted of protein precipitation. Separation was performed...

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Bibliographic Details
Published in:Biomedical chromatography 2024-07, Vol.38 (7), p.e5874-n/a
Main Authors: Hulskotte, Lotte M. G., Wilbrink‐Pijffers, Inge, Arbouw, Maurits E. L., Benoist, Guillemette E., Jansman, Frank G. A., Berlo‐van de Laar, Inge R. F.
Format: Article
Language:English
Subjects:
chromatography
drug monitoring
oxycodone
pharmacokinetics
tandem mass spectrometry
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Summary:A sensitive and reliable LC–MS/MS method was developed and validated for the quantification of oxycodone and metabolites in human plasma. The method has a runtime of 6 min and a sensitivity of 0.1 μg/L for all analytes. Sample preparation consisted of protein precipitation. Separation was performed on a Kinetix biphenyl column (2.1 × 100 mm, 1.7 μm), using ammonium formate 5 mm in 0.1% aqueous formic acid and methanol LC–MS grade 100% in gradient elution at a flow rate of 0.4 ml/min. Detection was performed in multiple reaction monitoring mode using positive electrospray ionization. The method was linear over the calibration range of 0.1–25.0 μg/L for oxycodone, noroxycodone and noroxymorphone and 0.1–5.0 μg/L for oxymorphone. The method demonstrated good performance in terms of intra‐ and interday accuracy (86.5–110.3%) and precision (CV 1.7–9.3%). The criteria for the matrix effect were met (CV 
ISSN:0269-3879
1099-0801
1099-0801
DOI:10.1002/bmc.5874