Development of polymorphic microsatellite markers for distylous–homostylous Primula secundiflora (Primulaceae) using HiSeq sequencing

Primula secundiflora is an insect-pollinated, perennial herb belonging to the section Proliferae (Primulaceae) that exhibits considerable variation in its mating system, with predominantly outcrossing populations comprising long-styled and short-styled floral morphs and selfing populations comprisin...

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Bibliographic Details
Published in:Genes & Genetic Systems 2024, Vol.99, pp.23-00340
Main Authors: Sun, Hua-Ying, Zhang, Wen-Ping, Zhou, Wei, Wu, Zhi-Kun, Zheng, Lan-Ping
Format: Article
Language:English
Subjects:
Alleles
distyly
Genetics, Population - methods
High-Throughput Nucleotide Sequencing - methods
homostyly
Microsatellite Repeats
microsatellites
Polymorphism, Genetic
Primula - genetics
Primula secundiflora
Primulaceae
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Summary:Primula secundiflora is an insect-pollinated, perennial herb belonging to the section Proliferae (Primulaceae) that exhibits considerable variation in its mating system, with predominantly outcrossing populations comprising long-styled and short-styled floral morphs and selfing populations comprising only homostyles. To facilitate future investigations of the population genetics and mating patterns of this species, we developed 25 microsatellite markers from P. secundiflora using next-generation sequencing and measured polymorphism and genetic diversity in a sample of 30 individuals from three natural populations. The markers displayed high polymorphism, with the number of observed alleles per locus ranging from three to 16 (mean = 8.36). The observed and expected heterozygosities ranged from 0.100 to 1.000 and 0.145 to 0.843, respectively. Twenty-one of the loci were also successfully amplified in P. denticulata. These microsatellite markers should provide powerful tools for investigating patterns of population genetic diversity and the evolutionary relationships between distyly and homostyly in P. secundiflora.
ISSN:1341-7568
1880-5779
DOI:10.1266/ggs.23-00340